Anticorps Monoclonal anti-SMARCA4

SMARCA4 Monoclonal Antibody for FC (Intra), IF

Hôte / Isotype

Mouse / IgG2b

Réactivité testée

Humain

Applications

IF, FC (Intra)

Conjugaison

CoraLite®594 Fluorescent Dye

CloneNo.

2E6B6

N° de cat : CL594-66561

Synonymes

ATP dependent helicase SMARCA4, BAF190, BAF190A, BRG1, BRG1 associated factor 190A, FLJ39786, hSNF2b, Protein brahma homolog 1, Protein BRG 1, SMARCA4, SNF2, SNF2 BETA, SNF2B, SNF2L4, SNF2LB, SWI2, Transcription activator BRG1

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Galerie de données de validation

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  IF Staining of HepG2 using CL594-66561

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CL594-66561 (SMARCA4 antibody) at dilution of 1:50.

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  IF Staining of HepG2 using CL594-66561

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CoraLite®594 SMARCA4 antibody (CL594-66561, Clone: 2E6B6 ) at dilution of 1:200. DAPI (blue).

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  FC experiment of HeLa using CL594-66561

  1X10^6 HeLa cells were intracellularly stained with 0.2 ug CoraLite®594 Anti-Human SMARCA4 (CL594-66561, Clone:2E6B6) (red), or 0.2 ug Mouse IgG2b Isotype Control (CL594-66360-3, Clone: K11B8C4B5) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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Applications testées

• Résultats positifs en IF: cellules HepG2,
• Résultats positifs en cytométrie: cellules HeLa,

Dilution recommandée

• Immunofluorescence (IF): IF : 1:50-1:500
• Flow Cytometry (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

CL594-66561 cible SMARCA4 dans les applications de IF, FC (Intra) et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Mouse / IgG2b
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: SMARCA4 Protéine recombinante Ag16256
• Nom complet: SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 4
• Masse moléculaire calculée: 1647 aa, 185 kDa
• Numéro d’acquisition GenBank: BC150298
• Symbole du gène: SMARCA4
• Identification du gène (NCBI): 6597
• Conjugaison: CoraLite®594 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 588 nm / 604 nm
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS avec glycérol à 50 %, Proclin300 à 0,05 % et BSA à 0,5 %, pH 7,3.
• Conditions de stockage: Stocker à -20 °C. Éviter toute exposition à la lumière. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

SMARCA4, also named as BAF190A, BRG1, SNF2B and SNF2L4, belongs to the SNF2/RAD54 helicase family. SMARCA4 is a transcriptional coactivator cooperating with nuclear hormone receptors to potentiate transcriptional activation. It is a component of the CREST-BRG1 complex, a multiprotein complex that regulates promoter activation by orchestrating a calcium-dependent release of a repressor complex and a recruitment of an activator complex. It is also involved in vitamin D-coupled transcription regulation via its association with the WINAC complex, a chromatin-remodeling complex recruited by vitamin D receptor (VDR), which is required for the ligand-bound VDR-mediated transrepression of the CYP27B1 gene. And this anitbody is CL594(Ex/Em 593 nm/614 nm) conjugated.

Produits associés

Foxp3 / Transcription Factor Staining Buffer Kit

PF00011