Anticorps Monoclonal anti-SMAD7
SMAD7 Monoclonal Antibody for WB, ELISA
Hôte / Isotype
Mouse / IgG2a
Réactivité testée
Humain, Lapin, porc, rat, souris
Applications
WB, ELISA
Conjugaison
Non conjugué
CloneNo.
3D1C3
N° de cat : 68594-1-Ig
Synonymes
Galerie de données de validation
Applications testées
Résultats positifs en WB | tissu rénal de porc, tissu cérébral de lapin, tissu cérébral de rat, tissu cérébral de souris |
Dilution recommandée
Application | Dilution |
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Western Blot (WB) | WB : 1:5000-1:50000 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, check data in validation data gallery |
Informations sur le produit
68594-1-Ig cible SMAD7 dans les applications de WB, ELISA et montre une réactivité avec des échantillons Humain, Lapin, porc, rat, souris
Réactivité | Humain, Lapin, porc, rat, souris |
Hôte / Isotype | Mouse / IgG2a |
Clonalité | Monoclonal |
Type | Anticorps |
Immunogène | SMAD7 Protéine recombinante Ag13688 |
Nom complet | SMAD family member 7 |
Masse moléculaire calculée | 426 aa, 46 kDa |
Poids moléculaire observé | 45-55 kDa |
Numéro d’acquisition GenBank | BC074819 |
Symbole du gène | SMAD7 |
Identification du gène (NCBI) | 4092 |
Conjugaison | Non conjugué |
Forme | Liquide |
Méthode de purification | Purification par protéine A |
Tampon de stockage | PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3 |
Conditions de stockage | Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20oC Les 20ul contiennent 0,1% de BSA. |
Informations générales
SMAD7, also named as Mothers against decapentaplegic homolog 7, is a 426 amino acid protein, which belongs to the dwarfin/SMAD family. SMAD7 Interaction with NEDD4L or RNF111 induces translocation from the nucleus to the cytoplasm (PubMed:16601693). TGF-beta stimulates its translocation from the nucleus to the cytoplasm. PDPK1 inhibits its translocation from the nucleus to the cytoplasm in response to TGF-beta (PubMed:17327236). SMAD7 as antagonist of signaling by TGF-beta type 1 receptor superfamily members has been shown to inhibit TGF-beta and activin signaling by associating with their receptors thus preventing SMAD2 access. SMAD7 functions as an adapter to recruit SMURF2 to the TGF-beta receptor complex and also acts by recruiting the PPP1R15A-PP1 complex to TGFBR1, which promotes its dephosphorylation. SMAD7 positively regulates PDPK1 kinase activity by stimulating its dissociation from the 14-3-3 protein YWHAQ which acts as a negative regulator.
Protocole
Product Specific Protocols | |
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WB protocol for SMAD7 antibody 68594-1-Ig | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |