Anticorps Polyclonal de lapin anti-SMAD3

SMAD3 Polyclonal Antibody for ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain et plus (2)

Applications

WB, ELISA

Conjugaison

Non conjugué

Publications

6

N° de cat : 51145-1-AP

Synonymes

hMAD 3, hSMAD3, HSPC193, HsT17436, JV15 2, MAD homolog 3, Mad3, MADH3, Mothers against DPP homolog 3, SMAD 3, SMAD family member 3, SMAD3

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Galerie de données de validation

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  WB analysis of HEK-293 using 51145-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 51145-1-AP (SMAD3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of human breast cancer using 51145-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 51145-1-AP (SMAD3 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human breast cancer using 51145-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 51145-1-AP (SMAD3 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human colon cancer using 51145-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 51145-1-AP (SMAD3 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human colon cancer using 51145-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 51145-1-AP (SMAD3 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human breast cancer using 51145-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 51145-1-AP (SMAD3 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human breast cancer using 51145-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 51145-1-AP (SMAD3 antibody) at dilution of 1:50 (under 40x lens).

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Applications testées

Dilution recommandée

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 6 publications below

Informations sur le produit

51145-1-AP cible SMAD3 dans les applications de WB, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: SMAD family member 3
• Masse moléculaire calculée: 48 kDa
• Numéro d’acquisition GenBank: BC050743
• Symbole du gène: SMAD3
• Identification du gène (NCBI): 4088
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20 ℃. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

SMAD family member 3 (SMAD3), also named Mothers against decapentaplegic homolog 3. Receptor-regulated SMAD (R-SMAD) that is an intracellular signal transducer and transcriptional modulator activated by TGF-beta (transforming growth factor) and activin type 1 receptor kinases. Binds the TRE element in the promoter region of many genes that are regulated by TGF-beta and, on formation of the SMAD3/SMAD4 complex, activates transcription. Also can form a SMAD3/SMAD4/JUN/FOS complex at the AP-1/SMAD site to regulate TGF-beta-mediated transcription. Has an inhibitory effect on wound healing probably by modulating both growth and migration of primary keratinocytes and by altering the TGF-mediated chemotaxis of monocytes. This effect on wound healing appears to be hormone-sensitive. Regulator of chondrogenesis and osteogenesis and inhibits early healing of bone fractures (By similarity). Positively regulates PDPK1 kinase activity by stimulating its dissociation from the 14-3-3 protein YWHAQ which acts as a negative regulator.The observed molecular weight of SMAD3 is about 48 kDa.

Publications

Species	Application	Title
mouse	WB	J Ethnopharmacol; Yishen Tongluo formula alleviates diabetic kidney disease through regulating Sirt6/TGF-β1/Smad2/3 pathway and promoting degradation of TGF-β1; Authors - Xiaowei Zhang
human	WB	Biomed Res Int; Multifunctional Drug-Loaded Phase-Change Nanoparticles Inhibit the Epithelial-Mesenchymal Transition of Hepatocellular Carcinoma by Affecting the Activity of Activated Hepatic Stellate Cells; Authors - Xiaomeng Zou
rat	WB	Dis Markers; Pyrrosia petiolosa Extract Ameliorates Ethylene Glycol-Induced Urolithiasis in Rats by Inhibiting Oxidative Stress and Inflammatory Response; Authors - Fangmin Zhou
rat	WB	Heliyon; Benzylisoquinoline alkaloids inhibit lung fibroblast activation mainly via inhibiting TGF-β1/Smads and ERK1/2 pathway proteins; Authors - Hui-Li Ren
human	WB	Pediatr Surg Int; Circular RNA MTCL1 targets SMAD3 by sponging miR-145-5p for regulation of cell proliferation and migration in Hirschsprung's disease; Authors - Wang Chen
human	WB	Int J Biol Sci; Hypoxia-driven TNS4 fosters HNSCC tumorigenesis by stabilizing integrin α5β1 complex and triggering FAK-mediated Akt and TGFβ signaling pathways; Authors - Xinyuan Zhao