Anticorps Polyclonal de lapin anti-SMAD4

• Phare
• Validé par KD/KO

SMAD4 Polyclonal Antibody for IHC, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, ELISA

Conjugaison

Non conjugué

Publications

3

N° de cat : 51144-1-AP

Synonymes

DPC4, hSMAD4, JIP, MADH4, Mothers against DPP homolog 4, SMAD 4, SMAD family member 4, SMAD4

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Galerie de données de validation

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  WB analysis of HeLa using 51144-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 51144-1-AP (SMAD4 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 cells using 51144-1-AP

  WB results of SMAD4 antibody (51144-1-AP, 1:1000) with si-Control and si-SMAD4 transfected HepG2 cells.

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  WB analysis of PC-3 using 51144-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 51144-1-AP (SMAD4 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of human placenta using 51144-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 51144-1-AP (SMAD4 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human placenta using 51144-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 51144-1-AP (SMAD4 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human heart using 51144-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 51144-1-AP (SMAD4 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human heart using 51144-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 51144-1-AP (SMAD4 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human heart using 51144-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 51144-1-AP (SMAD4 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human heart using 51144-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 51144-1-AP (SMAD4 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human heart using 51144-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 51144-1-AP (SMAD4 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human heart using 51144-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 51144-1-AP (SMAD4 antibody) at dilution of 1:50 (under 40x lens).

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules HepG2
• Résultats positifs en IHC: tissu placentaire humain, tissu cardiaque humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochimie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 3 publications below

Informations sur le produit

51144-1-AP cible SMAD4 dans les applications de WB, IHC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: SMAD family member 4
• Masse moléculaire calculée: 60 kDa
• Poids moléculaire observé: 58 kDa
• Numéro d’acquisition GenBank: BC002379
• Symbole du gène: SMAD4
• Identification du gène (NCBI): 4089
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20 ℃. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Mammalian homologs of the Drosophila Mad gene include Smad1, Smad2, Smad3, Smad4 (DPC4), Smad5, Smad6, Smad7 and Smad8. Smad1 and Smad5 are effectors of BMP2 and BMP4 function while Smad2 and Smad3 are involved in TGF and activin-mediated growth modulation. Smad4 has been shown to mediate all of the above activities through interactionwith various Smad family members . Smad6 and Smad7 regulate the response to activin/ TGF signaling by interfering with TGF -mediated phosphorylation of other Smad family members. This antibody is a rabbit polyclonal antibody raised against a peptide (25-43aa) mapping within human SMAD4. It is specific to SMAD4.

Publications

Species	Application	Title
rat	WB	Exp Ther Med; Naringin attenuates renal interstitial fibrosis by regulating the TGF-β/Smad signaling pathway and inflammation.; Authors - Ruichen Wang
rat	WB	Open Life Sci; NR4A1 inhibits the epithelial-mesenchymal transition of hepatic stellate cells: Involvement of TGF-β-Smad2/3/4-ZEB signaling.; Authors - Qian Huang
rat	WB	Exp Ther Med; MicroRNA-122 inhibits epithelial-mesenchymal transition of hepatic stellate cells induced by the TGF-β1/Smad signaling pathway.; Authors - Bianqiao Cheng