Anticorps Monoclonal anti-SLP76

SLP76 Monoclonal Antibody for FC (Intra), IF, WB, ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain

Applications

WB, IF, FC (Intra), ELISA

Conjugaison

Non conjugué

CloneNo.

4H10B1

N° de cat : 66465-1-Ig

Synonymes

LCP2, Lymphocyte cytosolic protein 2, SLP 76, SLP 76 tyrosine phosphoprotein, SLP76

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Galerie de données de validation

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  WB analysis of Jurkat using 66465-1-Ig

  Jurkat cells were subjected to SDS PAGE followed by western blot with 66465-1-Ig (SLP76 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of MOLT-4 using 66465-1-Ig

  MOLT-4 cells were subjected to SDS PAGE followed by western blot with 66465-1-Ig (SLP76 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of K-562 using 66465-1-Ig

  K-562 cells were subjected to SDS PAGE followed by western blot with 66465-1-Ig (SLP76 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IF Staining of HeLa using 66465-1-Ig

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using 66465-1-Ig (SLP76 antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

• 

  FC experiment of Jurkat using 66465-1-Ig

  1X10^6 Jurkat cells were intracellularly stained with 0.4 ug Anti-Human SLP76 (66465-1-Ig, Clone:4H10B1) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Applications testées

• Résultats positifs en WB: cellules Jurkat, cellules K-562, cellules MOLT-4
• Résultats positifs en IF: cellules HeLa,
• Résultats positifs en cytométrie: cellules Jurkat

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunofluorescence (IF): IF : 1:50-1:500
• Flow Cytometry (FC): FC : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

66465-1-Ig cible SLP76 dans les applications de WB, IF, FC (Intra), ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: SLP76 Protéine recombinante Ag3432
• Nom complet: lymphocyte cytosolic protein 2 (SH2 domain containing leukocyte protein of 76kDa)
• Masse moléculaire calculée: 533 aa, 60 kDa
• Poids moléculaire observé: 76 kDa
• Numéro d’acquisition GenBank: BC016618
• Symbole du gène: LCP2
• Identification du gène (NCBI): 3937
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

LCP2, also named as SLP76, is an adapter protein that acts as a substrate of the T cell antigen receptor (TCR)-activated protein tyrosine kinase pathway. It contains an N-terminal leucine Zip motif, a tyrosine-rich domain, a proline-rich domain and a C-terminal SH2 domain. It is constitutively associated with the growth factor receptor-bound protein 2 (Grb2)-related adapter protein 2 (Grap2, a.k.a. GADS) through the interaction of its proline residues with one of the SH3 domains of Grap2. SLP76 associates with growth factor receptor bound protein 2 (Grap2), and is thought to play a role TCR-mediated intracellular signal transduction. SLP76 is also expressed by mast cells, and mast cell activation was markedly impaired in SLP76-deficient mice.