Anticorps Polyclonal de lapin anti-Band 3/ AE1

Band 3/ AE1 Polyclonal Antibody for IF, IHC, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IP, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

4

N° de cat : 28131-1-AP

Synonymes

AE 1, AE1, Anion exchange protein 1, Anion exchanger 1, band 3, Band 3 anion transport protein, band 3/ AE1, CD233, SLC4A1

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Galerie de données de validation

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  WB analysis of COLO 320 using 28131-1-AP

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 28131-1-AP (band 3/ AE1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse liver using 28131-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 28131-1-AP (band 3/ AE1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of human spleen using 28131-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen tissue slide using 28131-1-AP (SLC4A1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human spleen using 28131-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen tissue slide using 28131-1-AP (band 3/ AE1antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human stomach cancer using 28131-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 28131-1-AP (band 3/ AE1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human stomach cancer using 28131-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 28131-1-AP (band 3/ AE1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of human placenta using 28131-1-AP

  Immunofluorescent analysis of (4% PFA) fixed human placenta tissue using band 3/ AE1 antibody (28131-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of human placenta using 28131-1-AP

  Immunofluorescent analysis of (4% PFA) fixed human placenta tissue using band 3/ AE1 antibody (28131-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules COLO 320, tissu hépatique de souris
• Résultats positifs en IHC: tissu splénique humain, tissu de cancer de l'estomac humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: tissu placentaire humain,

Dilution recommandée

• Western Blot (WB): WB : 1:200-1:1000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 1 publications below
• IF: See 2 publications below
• IP: See 1 publications below

Informations sur le produit

28131-1-AP cible Band 3/ AE1 dans les applications de WB, IP, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Band 3/ AE1 Protéine recombinante Ag27878
• Nom complet: solute carrier family 4, anion exchanger, member 1 (erythrocyte membrane protein band 3, Diego blood group)
• Masse moléculaire calculée: 911 aa, 102 kDa
• Poids moléculaire observé: 95-100 kDa
• Numéro d’acquisition GenBank: BC101570
• Symbole du gène: SLC4A1
• Identification du gène (NCBI): 6521
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Anion exchanger 1 (AE1), also known as band 3, is a 95-100 kDa transmembrane glycoprotein which is abundantly expressed in erythrocyte plasma membrane and mediates the electroneutral exchange of Cl- and HCO3-. It contains a 52-55 kDa C-terminal membrane crossing domain and a 43 kDa N-terminal cytoplasmic domain. Degradation of band 3 protein occurred during erythrocyte aging. The oxidative stress can also activate the proteolysis of band 3 through caspases.

Publications

Species	Application	Title
human	WB	Mol Cell; The mTORC1-SLC4A7 axis stimulates bicarbonate import to enhance de novo nucleotide synthesis.; Authors - Eunus S Ali
human	IP	Int J Hematol; Band 3 ectopic expression in colorectal cancer induces an increase in erythrocyte membrane-bound IgG and may cause immune-related anemia.; Authors - Akihito Kitao
mouse	IF	eLife; Piezo1 as a force-through-membrane sensor in red blood cells; Authors - George Vaisey
human	IF	NPJ Regen Med; Ferric citrate and apo-transferrin enable erythroblast maturation with β-globin from hemogenic endothelium; Authors - Soo-Been Jeon