Anticorps Monoclonal anti-SLC36A3

SLC36A3 Monoclonal Antibody for IF, IHC, WB, ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, souris

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

CloneNo.

1A8E6

N° de cat : 67929-1-Ig

Synonymes

FLJ16658, PAT3, SLC36A3, TRAMD2, Tramdorin 2, tramdorin2

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Galerie de données de validation

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  WB analysis of LNCaP using 67929-1-Ig

  LNCaP cells were subjected to SDS PAGE followed by western blot with 67929-1-Ig (SLC36A3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse testis using 67929-1-Ig

  unboiled mouse testis tissue were subjected to SDS PAGE followed by western blot with 67929-1-Ig (SLC36A3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of COLO 320 using 67929-1-Ig

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 67929-1-Ig (SLC36A3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Caco-2 using 67929-1-Ig

  Caco-2 cells were subjected to SDS PAGE followed by western blot with 67929-1-Ig (SLC36A3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 67929-1-Ig

  HepG2 cells were subjected to SDS PAGE followed by western blot with 67929-1-Ig (SLC36A3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of PC-3 using 67929-1-Ig

  PC-3 cells were subjected to SDS PAGE followed by western blot with 67929-1-Ig (SLC36A3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of mouse testis using 67929-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 67929-1-Ig (SLC36A3 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse testis using 67929-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 67929-1-Ig (SLC36A3 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of mouse testis using 67929-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed mouse testis tissue using SLC36A3 antibody (67929-1-Ig, Clone: 1A8E6 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

• 

  IF Staining of mouse testis using 67929-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed mouse testis tissue using SLC36A3 antibody (67929-1-Ig, Clone: 1A8E6 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules LNCaP, cellules Caco-2, cellules COLO 320, cellules HepG2, cellules PC-3, tissu testiculaire de souris non bouilli
• Résultats positifs en IHC: tissu testiculaire de souris, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: tissu testiculaire de souris,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:6000
• Immunohistochimie (IHC): IHC : 1:500-1:2000
• Immunofluorescence (IF): IF : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

67929-1-Ig cible SLC36A3 dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: SLC36A3 Protéine recombinante Ag16556
• Nom complet: solute carrier family 36 (proton/amino acid symporter), member 3
• Masse moléculaire calculée: 511 aa, 56 kDa
• Poids moléculaire observé: 46-51 kDa
• Numéro d’acquisition GenBank: BC101092
• Symbole du gène: SLC36A3
• Identification du gène (NCBI): 285641
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.