Anticorps Monoclonal anti-SLC31A1

• Phare
• Validé par KD/KO

SLC31A1 Monoclonal Antibody for FC, IF, IHC, WB, ELISA

Hôte / Isotype

Mouse / IgG2b

Réactivité testée

Humain

Applications

WB, IHC, IF, FC, ELISA

Conjugaison

Non conjugué

Publications

5

CloneNo.

1A4H5

N° de cat : 67221-1-Ig

Synonymes

Copper transporter 1, COPT1, CTR1, hCTR1, SLC31A1

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Galerie de données de validation

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  WB analysis of HepG2 using 67221-1-Ig

  HepG2 cells were subjected to SDS PAGE followed by western blot with 67221-1-Ig (SLC31A1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 67221-1-Ig

  HeLa cells were subjected to SDS PAGE followed by western blot with 67221-1-Ig (SLC31A1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 67221-1-Ig

  WB result of SLC31A1 antibody (67221-1-Ig; 1:12000; incubated at room temperature for 1.5 hours) with sh-Control and sh-SLC31A1 transfected HeLa cells.

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  IHC staining of human liver cancer using 67221-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67221-1-Ig (SLC31A1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 67221-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67221-1-Ig (SLC31A1 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 67221-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67221-1-Ig (SLC31A1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 67221-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67221-1-Ig (SLC31A1 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of human liver cancer using 67221-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using 67221-1-Ig (SLC31A1 antibody) at dilution of 1:100 and CoraLite488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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  IF Staining of human liver cancer using 67221-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using 67221-1-Ig (SLC31A1 antibody) at dilution of 1:100 and CoraLite488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

• 

  FC experiment of HeLa using 67221-1-Ig

  1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human SLC31A1 (67221-1-Ig, Clone:1A4H5) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Applications testées

• Résultats positifs en WB: cellules HepG2, cellules HeLa
• Résultats positifs en IHC: tissu de cancer du foie humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: tissu de cancer du foie humain,
• Résultats positifs en cytométrie: cellules HeLa,

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:20000
• Immunohistochimie (IHC): IHC : 1:1000-1:4000
• Immunofluorescence (IF): IF : 1:50-1:500
• Flow Cytometry (FC): FC : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 2 publications below
• WB: See 3 publications below
• IHC: See 2 publications below
• IF: See 1 publications below

Informations sur le produit

67221-1-Ig cible SLC31A1 dans les applications de WB, IHC, IF, FC, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain
• Hôte / Isotype: Mouse / IgG2b
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: SLC31A1 Protéine recombinante Ag28410
• Nom complet: solute carrier family 31 (copper transporters), member 1
• Masse moléculaire calculée: 21 kDa
• Poids moléculaire observé: 32 kDa
• Numéro d’acquisition GenBank: BC013611
• Symbole du gène: SLC31A1
• Identification du gène (NCBI): 1317
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Publications

Species	Application	Title
human	IHC	Front Mol Biosci; Significance of cuproptosis- related genes in the diagnosis and classification of psoriasis; Authors - Qingyuan Lin
human	WB	J Virol; Identification of Copper Transporter 1 as a Receptor for Feline Endogenous Retrovirus ERV-DC14.; Authors - Sandrine Tury ; KD Validated
human	IHC	Front Immunol; Identification of hub cuproptosis related genes and immune cell infiltration characteristics in periodontitis; Authors - Shuying Liu
human	WB	Cell Rep; A co-opted endogenous retroviral envelope promotes cell survival by controlling CTR1-mediated copper transport and homeostasis; Authors - Sandrine Tury ; KO Validated
human	WB,IF	Aging (Albany NY); Integrated bioinformatics and experiment revealed that cuproptosis is the potential common pathogenesis of three kinds of primary cardiomyopathy; Authors - Mengxi Wang