Anticorps Polyclonal de lapin anti-SLC31A1

• Phare
• Validé par KD/KO

SLC31A1 Polyclonal Antibody for FC, IF, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IF, FC, ELISA

Conjugaison

Non conjugué

Publications

2

N° de cat : 27499-1-AP

Synonymes

Copper transporter 1, COPT1, CTR1, hCTR1, SLC31A1

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Galerie de données de validation

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  WB analysis of HT-29 using 27499-1-AP

  HT-29 cells were subjected to SDS PAGE followed by western blot with 27499-1-AP (SLC31A1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 27499-1-AP

  WB result of SLC31A1 antibody (27499-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-SLC31A1 transfected HeLa cells.

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  WB analysis using 27499-1-AP

  Caco-2 cells were subjected to SDS PAGE followed by western blot with 27499-1-AP (SLC31A1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  IF Staining of human liver cancer using 27499-1-AP

  Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using SLC31A1 antibody (27499-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  FC experiment of Neuro-2a using 27499-1-AP

  1X10^6 Neuro-2a cells were intracellularly stained with 0.4 ug Anti-Human SLC31A1 (27499-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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  FC experiment of HeLa using 27499-1-AP

  1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human SLC31A1 (27499-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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  FC experiment of HeLa using 27499-1-AP

  1X10^6 HeLa cells were surface stained with 0.4 ug Anti-Human SLC31A1 (27499-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were not fixed .

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Applications testées

• Résultats positifs en WB: cellules HT-29, cellules Caco-2, cellules HeLa
• Résultats positifs en IF: tissu de cancer du foie humain,
• Résultats positifs en cytométrie: cellules HeLa, cellules Neuro-2a

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunofluorescence (IF): IF : 1:50-1:500
• Flow Cytometry (FC): FC : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 2 publications below

Informations sur le produit

27499-1-AP cible SLC31A1 dans les applications de WB, IF, FC, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: SLC31A1 Protéine recombinante Ag26601
• Nom complet: solute carrier family 31 (copper transporters), member 1
• Masse moléculaire calculée: 21 kDa
• Poids moléculaire observé: 21-25,30-35,60-70 kDa
• Numéro d’acquisition GenBank: BC013611
• Symbole du gène: SLC31A1
• Identification du gène (NCBI): 1317
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Publications

Species	Application	Title
	WB	Int J Mol Sci; Transcriptional Responses of Copper-Transport-Related Genes ctr1, ctr2 and atox1 and Their Roles in the Regulation of Cu Homeostasis in Yellow Catfish Pelteobagrus fulvidraco; Authors - Hong Yang
human	WB	Cancer Res Commun; Targeting NEDDylation is a Novel Strategy to Attenuate Cisplatin-induced Nephrotoxicity; Authors - Trace M Jones