Anticorps Monoclonal anti-SLC2A9

SLC2A9 Monoclonal Antibody for IF, IHC, WB, ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, Lapin, porc, rat, souris

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

4

CloneNo.

2D6E1

N° de cat : 67530-1-Ig

Synonymes

Glucose transporter type 9, GLUT 9, GLUT9, GLUTX, SLC2A9, UAQTL2, URATv1

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Galerie de données de validation

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  WB analysis using 67530-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67530-1-Ig (SLC2A9 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis using 67530-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67530-1-Ig (SLC2A9 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 67530-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67530-1-Ig (SLC2A9 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human liver using 67530-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 67530-1-Ig (SLC2A9 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human hepatocirrhosis using 67530-1-Ig

  Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis tissue slide using 67530-1-Ig (SLC2A9 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human hepatocirrhosis using 67530-1-Ig

  Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis tissue slide using 67530-1-Ig (SLC2A9 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver using 67530-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 67530-1-Ig (SLC2A9 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of human hepatocirrhosis using 67530-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed human hepatocirrhosis tissue using SLC2A9 antibody (67530-1-Ig, Clone: 2D6E1 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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  IF Staining of human kidney using 67530-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed human kidney tissue using SLC2A9 antibody (67530-1-Ig, Clone: 2D6E1 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

• 

  IF Staining of human kidney using 67530-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed human kidney tissue using SLC2A9 antibody (67530-1-Ig, Clone: 2D6E1 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

• 

  IF Staining of human hepatocirrhosis using 67530-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed human hepatocirrhosis tissue using SLC2A9 antibody (67530-1-Ig, Clone: 2D6E1 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules SMMC-7721, cellules HepG2, cellules HSC-T6, cellules HuH-7, cellules L02, tissu hépatique de lapin, tissu hépatique de porc, tissu hépatique de rat, tissu hépatique de souris
• Résultats positifs en IHC: tissu hépatique humain, tissu de cirrhose hépatique humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: tissu de cirrhose hépatique humain, tissu rénal humain

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochimie (IHC): IHC : 1:500-1:2000
• Immunofluorescence (IF): IF : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 3 publications below
• IHC: See 1 publications below

Informations sur le produit

67530-1-Ig cible SLC2A9 dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, Lapin, porc, rat, souris

• Réactivité: Humain, Lapin, porc, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: SLC2A9 Protéine recombinante Ag24135
• Nom complet: solute carrier family 2 (facilitated glucose transporter), member 9
• Masse moléculaire calculée: 540 aa, 59 kDa
• Poids moléculaire observé: 56-59 kDa
• Numéro d’acquisition GenBank: BC018897
• Symbole du gène: SLC2A9
• Identification du gène (NCBI): 56606
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Publications

Species	Application	Title
rat	IHC	Evid Based Complement Alternat Med; Effects of a Macroporous Resin Extract of Dendrobium officinale Leaves in Rats with Hyperuricemia Induced by Anthropomorphic Unhealthy Lifestyle; Authors - Lin-Zi Li
mouse	WB	J Pharm Pharmacol; Antihyperuricemic and nephroprotective effects of extracts from Orthosiphon stamineus in hyperuricemic mice.; Authors - Wen-Hao Xu
mouse	WB	Curr Res Food Sci; Effects of polysaccharides-riched Prunus mume fruit juice concentrate on uric acid excretion and gut microbiota in mice with adenine-induced chronic kidney disease; Authors - Yan Huang
human,mouse	WB	Biomed Pharmacother; Sanghuangporus vaninii ethanol extract alleviates hyperuricemic renal injury by regulating the uric acid transporters and inhibiting HK-2 apoptosis; Authors - Liping Hua