Anticorps Monoclonal anti-SLC25A1

SLC25A1 Monoclonal Antibody for WB, ELISA

Hôte / Isotype

Mouse / IgG3

Réactivité testée

Humain, rat, souris

Applications

WB, IP, ELISA

Conjugaison

Non conjugué

Publications

1

CloneNo.

1F11A3

N° de cat : 66771-1-Ig

Synonymes

Citrate transport protein, CTP, SLC20A3, SLC25A1, Tricarboxylate carrier protein

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Galerie de données de validation

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  WB analysis using 66771-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66771-1-Ig (SLC25A1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control.

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  WB analysis using 66771-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66771-1-Ig (SLC25A1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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Applications testées

• Résultats positifs en WB: cellules HEK-293, cellules HSC-T6, cellules Jurkat, cellules NIH/3T3

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 1 publications below
• IP: See 1 publications below

Informations sur le produit

66771-1-Ig cible SLC25A1 dans les applications de WB, IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: souris
• Hôte / Isotype: Mouse / IgG3
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: SLC25A1 Protéine recombinante Ag7520
• Nom complet: solute carrier family 25 (mitochondrial carrier; citrate transporter), member 1
• Masse moléculaire calculée: 34 kDa
• Poids moléculaire observé: 34 kDa
• Numéro d’acquisition GenBank: BC004980
• Symbole du gène: SLC25A1
• Identification du gène (NCBI): 6576
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

SLC25A1, also known as CIC or CTP, is a mitochondrial citrate transporter that exports citrate from the mitochondria to the cytosol. SLC25A1 is highly expressed in several tumor types. Recently it has been identified as a novel transcriptional target of mutant p53 and a negative tumor prognostic marker. In addition, defects in SLC25A1 has been found as a cause of combined D-2- and L-2-hydroxyglutaric aciduria. This antibody specifically recognized the endogenous SLC25A1; no protein was detected in cells from subjects containing two alleles with truncating mutations with this antibody. (24681808, 23561848)

Publications

Species	Application	Title
mouse	WB,IP	Nat Commun; IL-1R-IRAKM-Slc25a1 signaling axis reprograms lipogenesis in adipocytes to promote diet-induced obesity in mice.; Authors - Weiwei Liu