Anticorps Monoclonal anti-SFPQ

• Phare
• Validé par KD/KO

SFPQ Monoclonal Antibody for FC, IF, IHC, WB, ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF, FC, ELISA

Conjugaison

Non conjugué

Publications

1

CloneNo.

1G4A5

N° de cat : 67129-1-Ig

Synonymes

100 kDa DNA pairing protein, hPOMp100, POMP100, PSF, PTB associated splicing factor, SFPQ

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Galerie de données de validation

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  WB analysis using 67129-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67129-1-Ig (SFPQ antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  WB analysis using 67129-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67129-1-Ig (SFPQ antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 67129-1-Ig

  WB result of SFPQ antibody (67129-1-Ig; 1:120000; incubated at room temperature for 1.5 hours) with sh-Control and sh-SFPQ transfected HeLa cells.

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  WB analysis of HSC-T6 using 67129-1-Ig

  HSC-T6 cells were subjected to SDS PAGE followed by western blot with 67129-1-Ig (SFPQ antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  WB analysis of NIH/3T3 using 67129-1-Ig

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 67129-1-Ig (SFPQ antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 67129-1-Ig

  HeLa cells were subjected to SDS PAGE followed by western blot with 67129-1-Ig (SFPQ antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 67129-1-Ig

  Jurkat cells were subjected to SDS PAGE followed by western blot with 67129-1-Ig (SFPQ antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours.

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  WB analysis of PC-3 using 67129-1-Ig

  PC-3 cells were subjected to SDS PAGE followed by western blot with 67129-1-Ig (SFPQ antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 67129-1-Ig

  HeLa cells were subjected to SDS PAGE followed by western blot with 67129-1-Ig (SFPQ antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 67129-1-Ig

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 67129-1-Ig (SFPQ antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 67129-1-Ig

  Jurkat cells were subjected to SDS PAGE followed by western blot with 67129-1-Ig (SFPQ antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  IHC staining of human colon cancer using 67129-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67129-1-Ig (SFPQ antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung cancer using 67129-1-Ig

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 67129-1-Ig (SFPQ antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human pancreas cancer using 67129-1-Ig

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 67129-1-Ig (SFPQ antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 67129-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67129-1-Ig (SFPQ antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse stomach using 67129-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse stomach tissue slide using 67129-1-Ig (SFPQ antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of rat brain using 67129-1-Ig

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 67129-1-Ig (SFPQ antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of rat stomach using 67129-1-Ig

  Immunohistochemical analysis of paraffin-embedded rat stomach tissue slide using 67129-1-Ig (SFPQ antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of MCF-7 using 67129-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using SFPQ antibody (67129-1-Ig, Clone: 1G4A5 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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  IF Staining of HeLa using 67129-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using SFPQ antibody (67129-1-Ig, Clone: 1G4A5 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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  FC experiment of HeLa using 67129-1-Ig

  1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human SFPQ (67129-1-Ig, Clone:1G4A5) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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Applications testées

• Résultats positifs en WB: cellules U-251, cellules A431, cellules HEK-293, cellules HeLa, cellules HSC-T6, cellules Jurkat, cellules K-562, cellules LNCaP, cellules NIH/3T3, cellules PC-3
• Résultats positifs en IHC: tissu d'estomac de rat, tissu cérébral de rat, tissu cérébral de souris, tissu de cancer du côlon humain, tissu de cancer du pancréas humain, tissu de cancer du poumon humain, tissu d'estomac de souris; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HeLa, cellules MCF-7
• Résultats positifs en cytométrie: cellules HeLa,

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochimie (IHC): IHC : 1:2000-1:8000
• Immunofluorescence (IF): IF : 1:400-1:1600
• Flow Cytometry (FC): FC : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 1 publications below

Informations sur le produit

67129-1-Ig cible SFPQ dans les applications de WB, IHC, IF, FC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: SFPQ Protéine recombinante Ag7181
• Nom complet: splicing factor proline/glutamine-rich (polypyrimidine tract binding protein associated)
• Masse moléculaire calculée: 76 kDa
• Poids moléculaire observé: 90-100 kDa
• Numéro d’acquisition GenBank: BC051192
• Symbole du gène: SFPQ
• Identification du gène (NCBI): 6421
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

SFPQ, also named PSF, encodes a nuclear factor implicated in the splicing and regulation of gene expression. SFPQ probably forms a heteromer with NONO and participates in DNA pairing and DNA break repair program. Very recently SFPQ was identified as a downstream target of tau, complete nuclear depletion and cytoplasmic accumulation of SFPQ were shown in the neurons and astrocytes of brains with Alzheimer's disease (AD), more strikingly, reduced SFPQ levels may progress together with tau pathology, these observation strongly suggests the important role of SFPQ pathology in neurodegenerative diseases including AD. SFPQ encompasses 707 amino acids and has a molecular weight of 76 kDa, although it typically migrates on a sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) gel at an apparent molecular weight of ∼100 kDa. Proteolytic cleavage products of apparent molecular weights of 47 and 68 kDa, and an alternatively spliced form of 669 amino acids, have also been described in various cell types. (PMID: 25832716). Splicing Factor Proline and Glutamine rich (SFPQ) as the most significant intron-retaining transcript across diverse ALS-causing mutations (VCP, SOD1 and FUS). SFPQ protein binds extensively to its retained intron, which exhibits high cytoplasmic abundance in VCP mutation compared with controls. Crucially, the protein is less abundant in the nuclei of VCP mutation cultures and is ultimately lost from nuclei of MNs in mouse models (SOD1mu and VCP mutation transgenic mouse models) and human sporadic ALS post-mortem samples. In summary, our study implicates SFPQ IR and nuclear loss as general molecular hallmarks of familial and sporadic ALS.

Publications

Species	Application	Title
human	WB	Int J Mol Sci; SFPQ and Its Isoform as Potential Biomarker for Non-Small-Cell Lung Cancer; Authors - Libang Yang