Anticorps Monoclonal anti-14-3-3 Sigma

14-3-3 Sigma Monoclonal Antibody for FC, IF, IHC, WB,ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain et plus (1)

Applications

WB, IHC, IF, FC, ELISA

Conjugaison

Non conjugué

Publications

4

CloneNo.

3F10D9

N° de cat : 66251-1-Ig

Synonymes

14 3 3 protein sigma, HME1, SFN, stratifin, YWHAS

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Galerie de données de validation

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  WB analysis using 66251-1-Ig

  Various cell lysates were subjected to SDS PAGE followed by western blot with 66251-1-Ig (14-3-3 sigma antibody) at dilution of 1:7000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 66251-1-Ig

  HeLa cells were subjected to SDS PAGE followed by western blot with 66251-1-Ig (14-3-3 sigma antibody at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of MCF-7 using 66251-1-Ig

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 66251-1-Ig (14-3-3 sigma antibody at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of A431 using 66251-1-Ig

  A431 cells were subjected to SDS PAGE followed by western blot with 66251-1-Ig (14-3-3 sigma antibody at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  IHC staining of human skin using 66251-1-Ig

  Immunohistochemical analysis of paraffin-embedded human skin tissue slide using 66251-1-Ig (14-3-3 sigma antibody at dilution of 1:2000 (under 10x lens).

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  IHC staining of human skin using 66251-1-Ig

  Immunohistochemical analysis of paraffin-embedded human skin tissue slide using 66251-1-Ig (14-3-3 sigma antibody at dilution of 1:2000 (under 40x lens).

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  IHC staining of human breast hyperplasia using 66251-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast hyperplasia tissue slide using 66251-1-Ig (14-3-3 sigma antibody at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast hyperplasia using 66251-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast hyperplasia tissue slide using 66251-1-Ig (14-3-3 sigma antibody at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 66251-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66251-1-Ig (14-3-3 sigma antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung cancer using 66251-1-Ig

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66251-1-Ig (14-3-3 sigma antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of MCF-7 using 66251-1-Ig

  Immunofluorescent analysis of MCF-7 cells using 66251-1-Ig (14-3-3 sigma antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG (H+L).

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  FC experiment of A431 using 66251-1-Ig

  1X10^6 A431 cells were intracellularly stained with 0.2 ug Anti-Human 14-3-3 Sigma (66251-1-Ig, Clone:3F10D9) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (green), and 0.2 ug Control Antibody. Cells were fixed with 90% MeOH.

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Applications testées

• Résultats positifs en WB: cellules A431, cellules HeLa, cellules MCF-7
• Résultats positifs en IHC: tissu cutané humain, tissu de cancer du poumon humain, tissu de cancer du sein humain, tissu d'hyperplasie mammaire humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules MCF-7
• Résultats positifs en cytométrie: cellules A431,

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:14000
• Immunohistochimie (IHC): IHC : 1:200-1:8000
• Immunofluorescence (IF): IF : 1:20-1:200
• Flow Cytometry (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 1 publications below
• IHC: See 3 publications below
• IF: See 2 publications below

Informations sur le produit

66251-1-Ig cible 14-3-3 Sigma dans les applications de WB, IHC, IF, FC, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: 14-3-3 Sigma Protéine recombinante Ag12644
• Nom complet: stratifin
• Masse moléculaire calculée: 28 kDa
• Poids moléculaire observé: 28-30 kDa
• Numéro d’acquisition GenBank: BC002995
• Symbole du gène: SFN
• Identification du gène (NCBI): 2810
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

14-3-3 Sigma is a member 14-3-3 family of highly conserved proteins participating in diverse cellular processes including cell cycle progression. It is exclusively present in various epithelial cells, and alternatively named as human epithelial marker (HEM), or stratifin. Alteration of its expression has been linked to cancer.

Publications

Species	Application	Title
human,mouse	IF	Nat Cell Biol; Persistence of a regeneration-associated, transitional alveolar epithelial cell state in pulmonary fibrosis.; Authors - Yoshihiko Kobayashi
human	WB,IHC,IF	Mol Biotechnol; Stratifin (SFN) Regulates Cervical Cancer Cell Proliferation, Apoptosis, and Cytoskeletal Remodeling and Metastasis Progression Through LIMK2/Cofilin Signaling; Authors - Naiyi Du
human	IHC	Transl Androl Urol; Discovery of bladder cancer biomarkers in paired pre- and postoperative urine samples.; Authors - Xuechao Li
human	IHC	Environ Toxicol; Multidimensional progressive single-cell sequencing reveals cell microenvironment composition and cancer heterogeneity in lung cancer; Authors - Hua Chen