Anticorps Polyclonal de lapin anti-SETD2

• Phare
• Validé par KD/KO

SETD2 Polyclonal Antibody for IF, IHC, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain et plus (1)

Applications

WB, IHC, IF, CoIP, ELISA

Conjugaison

Non conjugué

Publications

5

N° de cat : 55377-1-AP

Synonymes

HIF 1, HIF1, HIP 1, hSET2, HSPC069, Huntingtin yeast partner B, HYPB, KIAA1732, KMT3A, Lysine N methyltransferase 3A, p231HBP, SET domain containing 2, SET2, SETD2

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Galerie de données de validation

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  WB analysis using 55377-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 55377-1-AP (SETD2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of U2OS cells using 55377-1-AP

  WB result of SETD2 antibody (55377-1-AP, 1:500) with siRNA transfected U2-OS cells.

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  WB analysis of Jurkat using 55377-1-AP

  WB result of SETD2 antibody (55377-1-AP; 1:400; room temperature for 1.5 hours) with wild-type and SETD2 knockout Jurkat cells.

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  IHC staining of human colon cancer using 55377-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 55377-1-AP (SETD2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human oesophagus cancer using 55377-1-AP

  Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using 55377-1-AP (SETD2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 55377-1-AP

  Immunofluorescent analysis of HepG2 cells using 55377-1-AP (SETD2 antibody) at dilution of 1:50 and Rhodamine-Goat anti-Rabbit IgG.

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Applications testées

• Résultats positifs en WB: cellules Jurkat, cellules K-562, cellules U2OS
• Résultats positifs en IHC: tissu de cancer du côlon humain, tissu de cancer de l'œsophage humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HepG2

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 2 publications below
• WB: See 4 publications below
• IHC: See 1 publications below
• CoIP: See 1 publications below

Informations sur le produit

55377-1-AP cible SETD2 dans les applications de WB, IHC, IF, CoIP, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: SET domain containing 2
• Masse moléculaire calculée: 288 kDa
• Poids moléculaire observé: 287 kDa
• Numéro d’acquisition GenBank: NM_014159
• Symbole du gène: SETD2
• Identification du gène (NCBI): 29072
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

SETD2, also named as HIF1, HYPB, KIAA1732, KMT3A, SET2, p231HBP, hSET2 and HIP-1, belongs to the histone-lysine methyltransferase family and SET2 subfamily. It is a histone methyltransferase that methylates 'Lys-36' of histone H3. SETD2 probably plays a role in chromatin structure modulation during elongation via its interaction with hyperphosphorylated POLR2A. It binds DNA at promoters. SETD2 may also act as a transcription activator that binds to promoters.(PMID:16118227) It is a potential tumour suppressor gene in breast cancer.(PMID:20944102) This antibody is specific to SETD2.

Publications

Species	Application	Title
human	WB	Nucleic Acids Res; PHD finger protein 1 (PHF1) is a novel reader for histone H4R3 symmetric dimethylation and coordinates with PRMT5-WDR77/CRL4B complex to promote tumorigenesis.; Authors - Ruiqiong Liu ; KD Validated
human,mouse	WB,IHC	Cell Death Dis; Knockdown of SETD2 promotes erastin-induced ferroptosis in ccRCC; Authors - Wei Xue ; KD Validated
human		Carcinogenesis; Proteomic analysis of the OGT interactome: novel links to epithelial-mesenchymal transition and metastasis of cervical cancer.; Authors - Jie Gao
human	WB	J Exp Clin Cancer Res; A novel FBW7/NFAT1 axis regulates cancer immunity in sunitinib-resistant renal cancer by inducing PD-L1 expression.; Authors - Wentao Liu
human	CoIP,WB	Function (Oxf); Structure and Function of RhoBTB1 Required for Substrate Specificity and Cullin-3 Ubiquitination; Authors - Gaurav Kumar