Anticorps Monoclonal anti-SET

SET Monoclonal Antibody for WB,ELISA

Hôte / Isotype

Mouse / IgG2b

Réactivité testée

Humain, rat, souris

Applications

WB, ELISA

Conjugaison

Non conjugué

Publications

1

CloneNo.

2B4D1

Avis (1)

N° de cat : 67478-1-Ig

Synonymes

2PP2A, HLA DR associated protein II, I 2PP2A, I2PP2A, IGAAD, IPP2A2, PHAPII, Protein SET, SET, SET nuclear oncogene, TAF I, TAF IBETA, Template activating factor I

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Galerie de données de validation

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  WB analysis of Jurkat using 67478-1-Ig

  Jurkat cells were subjected to SDS PAGE followed by western blot with 67478-1-Ig (SET antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HSC-T6 using 67478-1-Ig

  HSC-T6 cells were subjected to SDS PAGE followed by western blot with 67478-1-Ig (SET antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of 4T1 using 67478-1-Ig

  4T1 cells were subjected to SDS PAGE followed by western blot with 67478-1-Ig (SET antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 67478-1-Ig

  HeLa cells were subjected to SDS PAGE followed by western blot with 67478-1-Ig (SET antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 67478-1-Ig

  HepG2 cells were subjected to SDS PAGE followed by western blot with 67478-1-Ig (SET antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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Applications testées

• Résultats positifs en WB: cellules Jurkat, cellules 4T1, cellules HeLa, cellules HepG2, cellules HSC-T6

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:6000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 1 publications below

Informations sur le produit

67478-1-Ig cible SET dans les applications de WB, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain
• Hôte / Isotype: Mouse / IgG2b
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: SET nuclear oncogene
• Masse moléculaire calculée: 33 kDa
• Poids moléculaire observé: 33-36 kDa
• Numéro d’acquisition GenBank: NM_003011
• Symbole du gène: SET
• Identification du gène (NCBI): 6418
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20 ℃. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

SET, also named as IGAAD, I-2PP2A, TAF-I, IGAAD and PHAPII, belongs to the nucleosome assembly protein (NAP) family.SET is a multifunctional oncoprotein which is ubiquitously expressed in all kinds of cells. The SET protein participates in many cellular processes including cell cycle, cell migration, apoptosis, transcription, and DNA repair. The SET protein plays important roles in facilitating cell proliferation via many pathways that promote tumorigenesis and metastasis.

Publications

Species	Application	Title
human	WB	Cell Rep; A p53/LINC00324 positive feedback loop suppresses tumor growth by counteracting SET-mediated transcriptional repression; Authors - Ling Zhang

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Chantal (Verified Customer) (10-04-2022)

Specific band appeared after 3 minutes of exposure. it works on mouse and human cell lines

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: MEFs, Hela, HEK293FT

SET Antibody Western Blot validation (1:1000 dilution) in MEFs, Hela, HEK293FT (Cat no:67478-1-Ig)