Anticorps Monoclonal anti-SERPING1/C1 Inactivator

• Phare
• Validé par KD/KO

SERPING1/C1 Inactivator Monoclonal Antibody for IF, WB, ELISA

Hôte / Isotype

Mouse / IgG2a

Réactivité testée

Humain, porc, rat

Applications

WB, IF, ELISA

Conjugaison

Non conjugué

Publications

1

CloneNo.

1B11B11

N° de cat : 66882-1-Ig

Synonymes

C1 esterase inhibitor, C1 Inh, C1 inhibiting factor, C1IN, C1INH, C1NH, HAE1, HAE2, Plasma protease C1 inhibitor, Serpin G1, SERPING1, SERPING1/C1 Inactivator

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Galerie de données de validation

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  WB analysis using 66882-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66882-1-Ig (SERPING1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  IF Staining of HeLa using 66882-1-Ig

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using SERPING1/C1 Inactivator antibody (66882-1-Ig, Clone: 1B11B11 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red).

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  IF Staining of HepG2 using 66882-1-Ig

  Immunofluorescent analysis of (-20°C Methanol) fixed HepG2 cells using SERPING1/C1 Inactivator antibody (66882-1-Ig, Clone: 1B11B11 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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  IF Staining of HepG2 using 66882-1-Ig

  Immunofluorescent analysis of (-20°C Methanol) fixed HepG2 cells using SERPING1/C1 Inactivator antibody (66882-1-Ig, Clone: 1B11B11 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Applications testées

• Résultats positifs en WB: tissu plasmatique humain, plasma de porc, plasma humain, sérum de rat
• Résultats positifs en IF: cellules HeLa, cellules HepG2

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:8000
• Immunofluorescence (IF): IF : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 1 publications below

Informations sur le produit

66882-1-Ig cible SERPING1/C1 Inactivator dans les applications de WB, IF, ELISA et montre une réactivité avec des échantillons Humain, porc, rat

• Réactivité: Humain, porc, rat
• Réactivité citée: Humain
• Hôte / Isotype: Mouse / IgG2a
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: SERPING1/C1 Inactivator Protéine recombinante Ag16782
• Nom complet: serpin peptidase inhibitor, clade G (C1 inhibitor), member 1
• Masse moléculaire calculée: 500 aa, 55 kDa
• Poids moléculaire observé: 100 kDa
• Numéro d’acquisition GenBank: BC011171
• Symbole du gène: SERPING1
• Identification du gène (NCBI): 710
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

SERPING1, also known as C1 Inhibitor (C1INH), is a member of the Serine proteinase inhibitor family. Its main function is the inhibition of the complement system to prevent spontaneous activation. It inhibits activated C1r and C1s of the first complement component and thus regulates complement activation. Deficiency of this protein is associated with hereditary angioneurotic oedema (HANE). C1INH is the most heavily glycosylated plasma protein.

Publications

Species	Application	Title
human	WB	iScience; Targeting endometrial inflammation in intrauterine adhesion ameliorates endometrial fibrosis by priming MSCs to secrete C1INH; Authors - Simin Yao ; KD Validated