Anticorps Monoclonal anti-Serpin C1/Antithrombin III

Serpin C1/Antithrombin III Monoclonal Antibody for IF, IHC, WB, ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

2

CloneNo.

2B2E5

Avis (2)

N° de cat : 66052-1-Ig

Synonymes

Antithrombin, Antithrombin III, AT3, ATIII, Serpin C1, SERPINC1

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Galerie de données de validation

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  WB analysis of L02 using 66052-1-Ig

  L02 cells were subjected to SDS PAGE followed by western blot with 66052-1-Ig (Antithrombin III antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of human testis using 66052-1-Ig

  human testis tissue were subjected to SDS PAGE followed by western blot with 66052-1-Ig (Antithrombin III antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 66052-1-Ig

  human brain tissue were subjected to SDS PAGE followed by western blot with 66052-1-Ig (Antithrombin III antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat brain using 66052-1-Ig

  rat brain tissue were subjected to SDS PAGE followed by western blot with 66052-1-Ig (Antithrombin III antibody at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human liver cancer using 66052-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66052-1-Ig (Antithrombin III antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 66052-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66052-1-Ig (Antithrombin III antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver using 66052-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66052-1-Ig (Antithrombin III antibody at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human liver using 66052-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66052-1-Ig (Antithrombin III antibody at dilution of 1:200 (under 40x lens).

• 

  IF Staining of L02 using 66052-1-Ig

  Immunofluorescent analysis of (-20℃ Ethanol ) fixed L02 cells using 66052-1-Ig(Antithrombin III antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

• 

  IF Staining of HepG2 using 66052-1-Ig

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using Serpin C1/Antithrombin III antibody (66052-1-Ig, Clone: 2B2E5 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-phalloidin (red).

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Applications testées

• Résultats positifs en WB: cellules L02, tissu cérébral de rat, tissu cérébral humain, tissu testiculaire humain
• Résultats positifs en IHC: tissu de cancer du foie humain, tissu hépatique humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HepG2, cellules L02

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 1 publications below
• IF: See 1 publications below

Informations sur le produit

66052-1-Ig cible Serpin C1/Antithrombin III dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: Serpin C1/Antithrombin III Protéine recombinante Ag9880
• Nom complet: serpin peptidase inhibitor, clade C (antithrombin), member 1
• Masse moléculaire calculée: 464 aa, 53 kDa
• Poids moléculaire observé: 53 kDa
• Numéro d’acquisition GenBank: BC022309
• Symbole du gène: SERPINC1
• Identification du gène (NCBI): 462
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

SERPINC1, also named Antithrombin III or Antithrombin, is a plasma protease inhibitor and a member of the serpin superfamily. This protein inhibits thrombin as well as other activated serine proteases of the coagulation system, and it regulates the blood coagulation cascade. More than 120 mutations have been identified for this gene, many of which are known to cause antithrombin-III deficiency.

Publications

Species	Application	Title
human	IF	Matrix Biol; Proteome-wide and matrisome-specific atlas of the human ovary computes fertility biomarker candidates and open the way for precision oncofertility.; Authors - Emna Ouni
mouse	WB	Front Oncol; Synergistic effects of nab-PTX and anti-PD-1 antibody combination against lung cancer by regulating the Pi3K/AKT pathway through the Serpinc1 gene; Authors - Jun Zhang

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Iram (Verified Customer) (01-15-2021)

Antithrombin III Monoclonal antibody giving a very clear sharp bands

• Applications: Western Blot
• Primary Antibody Dilution: 1:2000 for western
• Cell Tissue Type: Human colon cancer cells

FH

Susmita (Verified Customer) (09-17-2020)

The antibody works good in mentioned dilution for WB and IHC

• Applications: Western Blot, Immunohistochemistry
• Primary Antibody Dilution: 1:1000 WB; 1:500 IHC

Serpin C1/Antithrombin III Antibody Western Blot,Immunohistochemistry validation (1:1000 WB; 1:500 IHC dilution) in (Cat no:66052-1-Ig)