Anticorps Monoclonal anti-SERCA2,ATP2A2

SERCA2,ATP2A2 Monoclonal Antibody for IHC, WB, ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

7

CloneNo.

1F11A11

Avis (2)

N° de cat : 67248-1-Ig

Synonymes

ATP2A2, ATP2B, Calcium pump 2, DAR, DD, DKFZp686P0211, FLJ20293, FLJ38063, SERCA2, SERCA2,ATP2A2, SERCA2b, SR Ca(2+) ATPase 2

---

Galerie de données de validation

• 

  WB analysis of HEK-293 using 67248-1-Ig

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 67248-1-Ig (SERCA2,ATP2A2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 67248-1-Ig

  HepG2 cells were subjected to SDS PAGE followed by western blot with 67248-1-Ig (SERCA2,ATP2A2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 67248-1-Ig

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 67248-1-Ig (SERCA2,ATP2A2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HSC-T6 using 67248-1-Ig

  HSC-T6 cells were subjected to SDS PAGE followed by western blot with 67248-1-Ig (SERCA2,ATP2A2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of NIH/3T3 using 67248-1-Ig

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 67248-1-Ig (SERCA2,ATP2A2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human urothelial carcinoma using 67248-1-Ig

  Immunohistochemical analysis of paraffin-embedded human urothelial carcinoma tissue slide using 67248-1-Ig (SERCA2,ATP2A2 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human urothelial carcinoma using 67248-1-Ig

  Immunohistochemical analysis of paraffin-embedded human urothelial carcinoma tissue slide using 67248-1-Ig (SERCA2,ATP2A2 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse skeletal muscle using 67248-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 67248-1-Ig (SERCA2,ATP2A2 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse skeletal muscle using 67248-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 67248-1-Ig (SERCA2,ATP2A2 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

---

Applications testées

• Résultats positifs en WB: cellules HEK-293, cellules HepG2, cellules HSC-T6, cellules MCF-7, cellules NIH/3T3
• Résultats positifs en IHC: tissu de carcinome urothélial humain, tissu de muscle squelettique de souris; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:3000-1:20000
• Immunohistochimie (IHC): IHC : 1:250-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 7 publications below
• IF: See 1 publications below

Informations sur le produit

67248-1-Ig cible SERCA2,ATP2A2 dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: SERCA2,ATP2A2 Protéine recombinante Ag5052
• Nom complet: ATPase, Ca++ transporting, cardiac muscle, slow twitch 2
• Masse moléculaire calculée: 1042 aa, 115 kDa
• Poids moléculaire observé: 120 kDa
• Numéro d’acquisition GenBank: BC035588
• Symbole du gène: ATP2A2
• Identification du gène (NCBI): 488
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

ATP2A2, also known as SERCA2 (Sarco/endoplasmic reticulum-type calcium transport ATPase 2), is a 110 kDa magnesium-dependent enzyme catalyzing the hydrolysis of ATP coupled with the translocation of calcium from the cytosol to the sarcoplasmic reticulum lumen. Human SERCA2 gene gives rise to several alternatively spliced isoforms: SERCA2a, SERCA2b and SERCA2c. ATP2A2 is mainly expressed in the sarcoplasmic reticulum of muscle cells and the endoplasmic reticulum of most cell types.

Publications

Species	Application	Title
mouse	WB	J Cell Physiol; microRNA-151-3p regulates slow muscle gene expression by targeting ATP2a2 in skeletal muscle cells.; Authors - Huan Wei
mouse	WB	Cell Calcium; Mannan-binding lectin deficiency augments hepatic endoplasmic reticulum stress through IP3R-controlled calcium release.; Authors - Yu Chen
rat	WB	Ecotoxicol Environ Saf; Fat mass and obesity-associated gene (FTO) hypermethylation induced by decabromodiphenyl ethane causing cardiac dysfunction via glucolipid metabolism disorder.; Authors - Leqiang Gao
human	WB	J Biol Chem; Cryo-EM structure of mouse TRPML2 in lipid nanodiscs; Authors - Xiaojing Song
human	WB	Clin Transl Med; Metabolic rescue ameliorates mitochondrial encephalo-cardiomyopathy in murine and human iPSC models of Leigh syndrome.; Authors - Jin‐Young Yoon
mouse	WB	Int J Biol Macromol; Lysine crotonylation of SERCA2a correlates to cardiac dysfunction and arrhythmia in Sirt1 cardiac-specific knockout mice; Authors - Huan-Xin Chen

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

---

FH

Susmita (Verified Customer) (06-13-2022)

This antibody is very good for WB analysis.

• Applications: Western Blot
• Primary Antibody Dilution: WB: 1: 2000
• Cell Tissue Type: PC cell line

FH

Iram (Verified Customer) (03-11-2022)

Very good antibody

• Applications: Western Blot
• Primary Antibody Dilution: 1:10000 for western
• Cell Tissue Type: Colon cancer cells