Anticorps Monoclonal anti-SDHA

SDHA Monoclonal Antibody for IF, IHC, WB,ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

5

CloneNo.

5E10G12

Avis (1)

N° de cat : 66588-1-Ig

Synonymes

FP, SDH2, SDHA, SDHF

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Galerie de données de validation

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  WB analysis using 66588-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66588-1-Ig (SDHA antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver cancer using 66588-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66588-1-Ig (SDHA antibody) at dilution of 1:2000 (under 10x lens).

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  IHC staining of human liver cancer using 66588-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66588-1-Ig (SDHA antibody) at dilution of 1:2000 (under 40x lens).

• 

  IF Staining of HepG2 using 66588-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 66588-1-Ig (SDHA antibody) at dilution of 1:100 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules HEK-293, cellules HepG2, cellules HSC-T6, cellules Jurkat, cellules NIH/3T3, cellules RAW 264.7
• Résultats positifs en IHC: tissu de cancer du foie humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HepG2,

Dilution recommandée

• Western Blot (WB): WB : 1:20000-1:80000
• Immunohistochimie (IHC): IHC : 1:1000-1:4000
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 4 publications below

Informations sur le produit

66588-1-Ig cible SDHA dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: SDHA Protéine recombinante Ag7023
• Nom complet: succinate dehydrogenase complex, subunit A, flavoprotein (Fp)
• Masse moléculaire calculée: 73 kDa
• Poids moléculaire observé: 62-73 kDa
• Numéro d’acquisition GenBank: BC001380
• Symbole du gène: SDHA
• Identification du gène (NCBI): 6389
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

SDHA, also named as SDH2, SDHF and Fp, belongs to the FAD-dependent oxidoreductase 2 family and FRD/SDH subfamily. SDHA is the flavoprotein subunit of succinate dehydrogenase (SDH) that is involved in complex II of the mitochondrial electron transport chain and is responsible for transferring electrons from succinate to ubiquinone (coenzyme Q). SDHA has 3 isoforms with the molecular mass of 73, 67 and 57 kDa, and the N-terminal (~5 kDa) can be removed in mature form. It can be detected a band of 68 kDa in cutaneous melanoma(PMID:21698147). SDHA may act as a tumor suppressor.

Publications

Species	Application	Title
human	WB	Nat Commun; Selective activator of human ClpP triggers cell cycle arrest to inhibit lung squamous cell carcinoma; Authors - Lin-Lin Zhou
		Sci China Life Sci; Cholesterol transport through the peroxisome-ER membrane contacts tethered by PI(4,5)P2 and extended synaptotagmins.; Authors - Jian Xiao
human,mouse	WB	Cell Chem Biol; Aberrant human ClpP activation disturbs mitochondrial proteome homeostasis to suppress pancreatic ductal adenocarcinoma.; Authors - Pengyu Wang
human	WB	Bioorg Chem; SAR-guided development of indole-matrine hybrids as potential anticancer agents via mitochondrial stress/cytochrome c/caspase 3 signaling pathway; Authors - Lingyu Li
mouse	WB	bioRxiv; FAM210A Regulates Mitochondrial Translation and Maintains Cardiac Mitochondrial Homeostasis; Authors - Jiangbin Wu

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Hua (Verified Customer) (07-12-2023)

Great antibody for WB. Very strong signal and clean background.

• Applications: Western Blot
• Primary Antibody Dilution: 1:10000
• Cell Tissue Type: Mouse liver

SDHA Antibody Western Blot validation (1:10000 dilution) in Mouse liver (Cat no:66588-1-Ig)