Anticorps Polyclonal de lapin anti-SARS-CoV-2 S protein (319-541 aa)

SARS-CoV-2 S protein (319-541 aa) Polyclonal Antibody for WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Virus

Applications

WB, ELISA

Conjugaison

Non conjugué

N° de cat : 28991-1-AP

Synonymes

SARS-CoV-2 Spike Glycoprotein (319-541 aa)

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Galerie de données de validation

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  WB analysis using 28991-1-AP

  E.coli expressed SARS-CoV-2 spike protein (Cat.NO. Ag30688) was subjected to SDS PAGE followed by western blot with 28991-1-AP (SARS-CoV-2 S protein (319-541 aa) antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  ELISA experiment of Recombinant protein using 28991-1-AP

  SARS-CoV-2 Spike Glycoprotein Antibody (28991-1-AP) tested by ELISA. SARS-CoV-2 Spike Glycoprotein (319-541 aa) were coated onto microtiter plates at 0.15 µg/well and then incubated with a dilution series of SARS-CoV-2 Spike Glycoprotein Antibody (28991-1-AP). Bound antibodies were detected with HRP conjugated Goat anti-Rabbit IgG followed by incubation with HRP Substrate and teminated with H2SO4, then measuring the resulting absorbance at 450 nm.

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Applications testées

• Résultats positifs en WB: Protéine recombinante,
• Résultats positifs en ELISA: Protéine recombinante,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000
• Test immuno-enzymatique (ELISA): ELISA : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

28991-1-AP cible SARS-CoV-2 S protein (319-541 aa) dans les applications de WB, ELISA et montre une réactivité avec des échantillons Virus

• Réactivité: Virus
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: SARS-CoV-2 S protein (319-541 aa) Protéine recombinante Ag30689
• Nom complet: SARS-CoV-2 Spike Protein
• Masse moléculaire calculée: 141 kDa
• Numéro d’acquisition GenBank: NC_045512
• Symbole du gène: COVID-19 S Protein
• Identification du gène (NCBI): 43740568
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Coronaviruses (CoVs) infect human and animals and cause varieties of diseases, including respiratory, enteric, renal, and neurological diseases. CoV uses its spike protein to recognize ACE2 as its receptors and mediate membrane fusion and virus entry into host cells(PMID: 32221306). Each monomer of trimeric S protein is about 180 kDa, and contains two subunits, S1 and S2,S1 recognizes and binds to host receptors, and subsequent conformational changes in S2 facilitate fusion between the viral envelope and the host cell membrane(PMID: 19198616). Although the amino acid sequences of the S-glycoprotein were found to be different between the various HCoV, the structures showed high similarity, but the best 3D structural overlap shared by SARS-CoV and SARS-CoV-2, consistent with the shared ACE2 predicted receptor (PMID: 32522207). The spike protein of CoVs can be a target for vaccine and therapeutic development (PMID: 19198616). 28991-1-AP is specific for spike protein of SARS-COV-2, that antigen region is 319-541aa.