Anticorps Polyclonal de lapin anti-S1PR1/EDG1

• Phare
• Validé par KD/KO

S1PR1/EDG1 Polyclonal Antibody for IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

29

N° de cat : 55133-1-AP

Synonymes

CD363, CHEDG1, D1S3362, ECGF1, EDG 1, EDG1, S1P receptor 1, S1P receptor Edg 1, S1P1, S1PR1, S1PR1/EDG1

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Galerie de données de validation

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  WB analysis using 55133-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 55133-1-AP (S1PR1/EDG1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse brain using 55133-1-AP

  IP Result of anti-S1PR1/EDG1 (IP:55133-1-AP, 4ug; Detection:55133-1-AP 1:300) with mouse brain tissue lysate 2640ug.

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Applications testées

• Résultats positifs en WB: tissu cérébral de souris, tissu cérébral de rat
• Résultats positifs en IP: tissu cérébral de souris

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:6000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 4 publications below
• WB: See 26 publications below
• IHC: See 6 publications below
• IF: See 5 publications below
• IP: See 2 publications below

Informations sur le produit

55133-1-AP cible S1PR1/EDG1 dans les applications de WB, IP, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: sphingosine-1-phosphate receptor 1
• Masse moléculaire calculée: 43 kDa
• Poids moléculaire observé: 40-50 kDa
• Numéro d’acquisition GenBank: NM_001400
• Symbole du gène: S1PR1
• Identification du gène (NCBI): 1901
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

S1PR1 (sphingosine-1-phosphate receptor 1), also known as S1P1 or EDG1, is one of five G-protein-coupled receptors for sphingosine-1-phosphate (S1P) (PMID: 18787560). S1P1 and its receptors have important regulatory functions in normal physiology and disease processes, particularly involving the immune, central nervous, and cardiovascular systems (PMID: 21339489). S1PR1 is widely expressed in various cell types, including endothelial cells and lymphocytes (PMID: 14737169). S1P-S1PR1 plays a major role in lymphocyte egress and chemotaxis, cell proliferation and survival, and tumor angiogenesis and metastasis (PMID: 21102457). The antibody is specific to S1PR1.

Publications

Species	Application	Title
mouse	WB	iScience; Global sphingosine-1-phosphate receptor 2 deficiency attenuates neuroinflammation and ischemic-reperfusion injury after neonatal stroke; Authors - Yumi Fukuzaki
rat	WB	Int Immunopharmacol; Dexmedetomidine alleviates oxidative stress and mitochondrial dysfunction in diabetic peripheral neuropathy via the microRNA-34a/SIRT2/S1PR1 axis; Authors - Ying Lin
human	IHC, WB, IP	Front Cell Dev Biol; The CAGE-MiR-181b-5p-S1PR1 Axis Regulates Anticancer Drug Resistance and Autophagy in Gastric Cancer Cells.; Authors - Minjeong Yeon
rat	WB	Front Pharmacol; Integrated Metabolomics and Network Pharmacology Approach to Explain Possible Action Mechanisms of Xin-Sheng-Hua Granule for Treating Anemia.; Authors - Han-Qing Pang
human,rat	WB,IHC	Phytomedicine; Geniposide alleviates VEGF-induced angiogenesis by inhibiting VEGFR2/PKC/ERK1/2-mediated SphK1 translocation.; Authors - Yan Wang
mouse	WB	Front Pharmacol; Coix Seed Oil Exerts an Anti-Triple-Negative Breast Cancer Effect by Disrupting miR-205/S1PR1 Axis.; Authors - Ting Fang