Anticorps Monoclonal anti-S100 Beta
S100 Beta Monoclonal Antibody for IF, IHC, WB, ELISA
Hôte / Isotype
Mouse / IgG1
Réactivité testée
Humain, porc, rat, souris
Applications
WB, IHC, IF, ELISA
Conjugaison
Non conjugué
CloneNo.
1G10F4
N° de cat : 66616-1-Ig
Synonymes
Galerie de données de validation
Applications testées
| Résultats positifs en WB | tissu cérébral de rat, cellules C6, tissu cérébral de porc |
| Résultats positifs en IHC | tissu de mélanome malin humain, tissu cérébral de rat, tissu cérébral de souris, tissu d'appendicite humain il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0. |
| Résultats positifs en IF | tissu cérébral de rat, |
Dilution recommandée
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunohistochimie (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF) | IF : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, check data in validation data gallery | |
Applications publiées
| WB | See 4 publications below |
| IHC | See 2 publications below |
| IF | See 10 publications below |
Informations sur le produit
66616-1-Ig cible S100 Beta dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, porc, rat, souris
| Réactivité | Humain, porc, rat, souris |
| Réactivité citée | rat, Humain, souris |
| Hôte / Isotype | Mouse / IgG1 |
| Clonalité | Monoclonal |
| Type | Anticorps |
| Immunogène | S100 Beta Protéine recombinante Ag7440 |
| Nom complet | S100 calcium binding protein B |
| Masse moléculaire calculée | 11 kDa |
| Poids moléculaire observé | 11 kDa |
| Numéro d’acquisition GenBank | BC001766 |
| Symbole du gène | S100B |
| Identification du gène (NCBI) | 6285 |
| Conjugaison | Non conjugué |
| Forme | Liquide |
| Méthode de purification | Purification par protéine G |
| Tampon de stockage | PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3 |
| Conditions de stockage | Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20oC Les 20ul contiennent 0,1% de BSA. |
Informations générales
S100B is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs and has been implicated in the regulation of cellular activities such as metabolism, motility and proliferation. In the nervous system, S100B is constitutively released by astrocytes into the extracellular space and at nanomolar concentrations, it can promote neurite outgrowth and protect neurons against oxidative stress. Within the central nervous system (CNS), S100B is thought to be a marker for astroglial activation, linking astrocyte dysfunction to schizophrenia. In addition to astrocytes, S100B is released from many cell types, such as, adipocytes, chondrocytes, cardiomyocytes and lymphocytes. Serum S100B represents a new biomarker for mood disorders.
Protocole
| Product Specific Protocols | |
|---|---|
| WB protocol for S100 Beta antibody 66616-1-Ig | Download protocol |
| IHC protocol for S100 Beta antibody 66616-1-Ig | Download protocol |
| IF protocol for S100 Beta antibody 66616-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Acta Neuropathol Commun Repetitive mild TBI causes pTau aggregation in nigra without altering preexisting fibril induced Parkinson’s-like pathology burden | ||
Cell Mol Life Sci Adtrp regulates thermogenic activity of adipose tissue via mediating the secretion of S100b. | ||
Exp Neurol Interaction of DCF1 with ATP1B1 induces impairment in astrocyte structural plasticity via the P38 signaling pathway. | ||
Front Pharmacol Delivery of Basic Fibroblast Growth Factor Through an In Situ Forming Smart Hydrogel Activates Autophagy in Schwann Cells and Improves Facial Nerves Generation via the PAK-1 Signaling Pathway. | ||
ACS Omega Platelet-Rich Plasma Gel-Loaded Collagen/Chitosan Composite Film Accelerated Rat Sciatic Nerve Injury Repair | ||
Front Neurosci Nogo-C Inhibits Peripheral Nerve Regeneration by Regulating Schwann Cell Apoptosis and Dedifferentiation. |
Avis
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH Clarisse (Verified Customer) (08-08-2022) | This antibody works very well as an astrocyte marker. In the image: in green mouse anti-S100B (1:500 - 66616-1-Ig) and in magenta rabbit anti-S100B (1:500 - 15146-1-AP). The rabbit antibody has a better performance.
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