Anticorps Monoclonal anti-S100A6

S100A6 Monoclonal Antibody for IF, IHC, WB, ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

1

CloneNo.

3D11C8

N° de cat : 66098-1-Ig

Synonymes

2A9, 5B10, CABP, CACY, Calcyclin, MLN 4, PRA, Protein S100 A6, S100A6

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Galerie de données de validation

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  WB analysis of HeLa using 66098-1-Ig

  HeLa cells were subjected to SDS PAGE followed by western blot with 66098-1-Ig (S100A6 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of A549 using 66098-1-Ig

  A549 cells were subjected to SDS PAGE followed by western blot with 66098-1-Ig (S100A6 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human colon cancer using 66098-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66098-1-Ig (S100A6 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 66098-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66098-1-Ig (S100A6 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human pancreas cancer using 66098-1-Ig

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 66098-1-Ig (S100A6 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human pancreas cancer using 66098-1-Ig

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 66098-1-Ig (S100A6 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 66098-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer slide using 66098-1-Ig (S100A6 Antibody) at dilution of 1:50.

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  IHC staining of human liver cancer using 66098-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer slide using 66098-1-Ig (S100A6 Antibody) at dilution of 1:50.

• 

  IHC staining of human colon cancer using 66098-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon cancer slide using 66098-1-Ig (S100A6 Antibody) at dilution of 1:50.

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  IHC staining of human colon cancer using 66098-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon cancer slide using 66098-1-Ig (S100A6 Antibody) at dilution of 1:50.

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  IF Staining of HepG2 using 66098-1-Ig

  Immunofluorescent analysis of HepG2 cells, using S100A6 antibody 66098-1-Ig at 1:25 dilution and Rhodamine-labeled goat anti-mouse IgG (red).

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules A549
• Résultats positifs en IHC: tissu de cancer du côlon humain, tissu de cancer du foie humain, tissu de cancer du pancréas humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HepG2

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 1 publications below

Informations sur le produit

66098-1-Ig cible S100A6 dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: S100A6 Protéine recombinante Ag17606
• Nom complet: S100 calcium binding protein A6
• Masse moléculaire calculée: 10 kDa
• Poids moléculaire observé: 10 kDa
• Numéro d’acquisition GenBank: BC001431
• Symbole du gène: S100A6
• Identification du gène (NCBI): 6277
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

S100A6 is also named as calcyclin, prolactin receptor-associated protein (PRA), growth factor-inducible protein 2A9 ir MLN4. It belongs to S100 family of low molecular weight, acidic, calcium-binding proteins which contain two EF-hand calcium binding sites. S100A6 may function as a calcium sensor to activate several processes in the calcium signal transduction pathway of cell growth, proliferation, secretion and exocytosis.

Publications

Species	Application	Title
human	WB	Open Med (Wars); S100A6 promotes proliferation and migration of HepG2 cells via increased ubiquitin-dependent degradation of p53.; Authors - Dongqiang Song