Anticorps Monoclonal anti-S100A11

• Phare
• Validé par KD/KO

S100A11 Monoclonal Antibody for IF, IHC, WB, ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain et plus (1)

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

5

CloneNo.

1A3F1

N° de cat : 60024-1-Ig

Synonymes

Calgizzarin, MLN 70, MLN70, Protein S100 A11, Protein S100 C, S100A11, S100C

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Galerie de données de validation

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  WB analysis of T-47D using 60024-1-Ig

  T-47D cells were subjected to SDS PAGE followed by western blot with 60024-1-Ig (S100A11 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of BxPC-3 using 60024-1-Ig

  BxPC-3 cells were subjected to SDS PAGE followed by western blot with 60024-1-Ig (S100A11 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of DU 145 using 60024-1-Ig

  DU 145 cells were subjected to SDS PAGE followed by western blot with 60024-1-Ig (S100A11 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of A431 using 60024-1-Ig

  A431 cells were subjected to SDS PAGE followed by western blot with 60024-1-Ig (S100A11 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human lung cancer using 60024-1-Ig

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 60024-1-Ig (S100A11 antibody) at dilution of 1:1000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung cancer using 60024-1-Ig

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 60024-1-Ig (S100A11 antibody) at dilution of 1:1000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human ovary tumor using 60024-1-Ig

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 60024-1-Ig (S100A11 antibody) at dilution of 1:1000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human ovary tumor using 60024-1-Ig

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 60024-1-Ig (S100A11 antibody) at dilution of 1:1000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human thyroid cancer using 60024-1-Ig

  Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 60024-1-Ig (S100A11 antibody) at dilution of 1:1000 (under 10x lens) Heat mediated antigen retrieved with Sodium Citrate buffer (pH 6.0).

• 

  IHC staining of human thyroid cancer using 60024-1-Ig

  Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 60024-1-Ig (S100A11 antibody) at dilution of 1:1000 (under 40x lens) Heat mediated antigen retrieved with Sodium Citrate buffer (pH 6.0).

• 

  IF Staining of BxPC-3 using 60024-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed BxPC-3 cells using S100A11 antibody (60024-1-Ig, Clone: 1A3F1 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules T-47D, cellules A431, cellules BxPC-3, cellules DU 145
• Résultats positifs en IHC: tissu de cancer du poumon humain, tissu de cancer de la thyroïde humain, tissu de tumeur ovarienne humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules BxPC-3,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:3000
• Immunohistochimie (IHC): IHC : 1:500-1:1000
• Immunofluorescence (IF): IF : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 3 publications below
• IF: See 1 publications below

Informations sur le produit

60024-1-Ig cible S100A11 dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: S100A11 Protéine recombinante Ag0343
• Nom complet: S100 calcium binding protein A11
• Masse moléculaire calculée: 105 aa, 12 kDa
• Poids moléculaire observé: 12 kDa
• Numéro d’acquisition GenBank: BC001410
• Symbole du gène: S100A11
• Identification du gène (NCBI): 6282
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

S100A11, also named as MLN 70, Calgizzarin and S100C, belongs to the S-100 family. It facilitates the differentiation and the cornification of keratinocytes. The naming of S100A11 systematically arises from its membership of the S100 protein family, named after their solubility in 100% saturated ammonium sulfate solution. First discovered in 1989, S100A11 has since been proposed to have direct biological functions in an assortment of physiological processes such as endo- and exocytosis, regulation of enzyme activity, cell growth and regulation, apoptosis and inflammation.

Publications

Species	Application	Title
human		Mol Cell Proteomics; S100A11 mediates hypoxia-induced mitogenic factor (HIMF)-induced smooth muscle cell migration, vesicular exocytosis, and nuclear activation.; Authors - Fan Chunling C ; KD Validated
human	WB	Talanta; Effective enrichment of cholangiocarcinoma secretomes using the hollow fiber bioreactor culture system.; Authors - Weeraphan Churat C
human	WB	Mol Cells; Proteomic analysis of the triglyceride-rich lipoprotein-laden foam cells.; Authors - Lu Yanjun Y
human	IF	Nat Commun; Annexin A1 is a polarity cue that directs mitotic spindle orientation during mammalian epithelial morphogenesis; Authors - Maria Fankhaenel
mouse	WB	Phytomedicine; Differences in p38-STAT3-S100A11 signaling after the administration of aristolochic acid I and IVa may account for the disparity in their nephrotoxicity; Authors - Zhong Xian