Anticorps Polyclonal de lapin anti-RanBP1

RanBP1 Polyclonal Antibody for IF, IHC, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

2

N° de cat : 27804-1-AP

Synonymes

HTF9A, RAN binding protein 1, RANBP1

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Galerie de données de validation

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  WB analysis using 27804-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 27804-1-AP (RanBP1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis using 27804-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 27804-1-AP (RanBP1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 27804-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 27804-1-AP (RanBP1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of fetal human brain using 27804-1-AP

  fetal human brain tissue were subjected to SDS PAGE followed by western blot with 27804-1-AP (RanBP1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse testis using 27804-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 27804-1-AP (RanBP1 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 27804-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using RanBP1 antibody (27804-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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  IF Staining of NIH/3T3 using 27804-1-AP

  Immunofluorescent analysis of (4% PFA) fixed NIH/3T3 cells using RanBP1 antibody (27804-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: tissu testiculaire de souris, cellules 3T3-L1, cellules HEK-293, cellules HeLa, cellules NIH/3T3, cellules RAW 264.7, tissu cérébral de souris, tissu cérébral humain fœtal
• Résultats positifs en IHC: tissu testiculaire de souris, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules NIH/3T3, cellules HeLa

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:6000
• Immunohistochimie (IHC): IHC : 1:400-1:1600
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 1 publications below
• IF: See 1 publications below

Informations sur le produit

27804-1-AP cible RanBP1 dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: RanBP1 Protéine recombinante Ag25714
• Nom complet: RAN binding protein 1
• Poids moléculaire observé: 28 kDa
• Symbole du gène: RANBP1
• Identification du gène (NCBI): 5902
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

RanBP1 (Ran-binding protein 1) belongs to the RANBP1 family. RanBP1 plays a role in RAN-dependent nucleocytoplasmic transport. It alleviates the TNPO1-dependent inhibition of RAN GTPase activity and mediates the dissociation of RAN from proteins involved in transport into the nucleus. And RanBP1 induces a conformation change in the complex formed by XPO1 and RAN that triggers the release of the nuclear export signal of cargo proteins (PMID:20485264). RanBP1 promotes dissociation of RAN from a complex with KPNA2 and CSE1L. It required for normal mitotic spindle assembly and normal progress through mitosis via its effect on RAN (PMID:17671426). Also, RanBP1 inhibits RCC1-dependent exchange of RAN-bound GDP by GTP (PMID:7882974, PMID:7616957).

Publications

Species	Application	Title
human	IF	J Med Chem; Structure-Guided Design of the First Noncovalent Small-Molecule Inhibitor of CRM1.; Authors - Yuqin Lei
human	WB	Elife; Distinct RanBP1 nuclear export and cargo dissociation mechanisms between fungi and animals.; Authors - Yuling Li