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Anticorps Polyclonal de lapin anti-Rabbit IgG control

Rabbit IgG control Polyclonal Antibody for FC

Hôte / Isotype

Lapin / IgG

Réactivité testée

Applications

FC

Conjugaison

FITC Fluorescent Dye

N° de cat : FITC-30000

Synonymes

IgG, IgG control, Isotype control, Rabbit IgG, Rabbit IgG isotype, Rabbit Immunoglobulin

Galerie de données de validation

Filter:
  • Flow cytometry (FC) experiment of HeLa cells using FITC-conjugated Rabbit IgG control Polyclonal anti (FITC-30000)

    FC experiment of HeLa using FITC-30000

    1X10^6 HeLa cells were intracellularly stained with 0.5 ug FITC Anti-Rabbit IgG control (FITC-30000) (red), or 0.5 ug FITC Anti-Human ATF4 (FITC-10835) (green), or unstained (blue). Cells were fixed with 4% PFA and permeabilized with Intracellular Staining Permeabilization Wash Buffer.

Applications testées

Résultats positifs en cytométrie

Dilution recommandée

ApplicationDilution
Flow Cytometry (FC)FC : 0.50 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Informations sur le produit

FITC-30000 cible Rabbit IgG control dans les applications de FC et montre une réactivité avec des échantillons

Réactivité
Hôte / Isotype Lapin / IgG
Clonalité Polyclonal
Type Anticorps
Immunogène s/o
Nom complet Rabbit IgG control
Symbole du gène
Identification du gène (NCBI)
Conjugaison FITC Fluorescent Dye
Excitation/Emission maxima wavelengths494 nm / 520 nm
Forme Liquide
Méthode de purification N/A
Tampon de stockage PBS avec glycérol à 50 %, Proclin300 à 0,05 % et BSA à 0,5 %, pH 7,3.
Conditions de stockageStocker à -20 °C. Éviter toute exposition à la lumière. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20oC Les 20ul contiennent 0,1% de BSA.

Informations générales

This product is Normal rabbit IgG (without immunized) which purified with Protein A. Normal Rabbit IgG is an isotype control antibody, which is used to estimate the non-specific binding of target primary antibodies due to Fc receptor binding or other protein-protein interactions. An isotype control antibody should have the same immunoglobulin type and be used at the same concentration as the test antibody.

Publications

SpeciesApplicationTitle

Virulence

Exploring the modulatory impact of isosakuranetin on Staphylococcus aureus: Inhibition of sortase A activity and α-haemolysin expression

Authors - Lili Tian
View Article