Anticorps Polyclonal de lapin anti-RSAD2

RSAD2 Polyclonal Antibody for IHC, WB,ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris et plus (1)

Applications

WB, IP, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

12

N° de cat : 28089-1-AP

Synonymes

2510004L01Rik, cig33, cig5, RSAD2, vig1, Viperin

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Galerie de données de validation

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  WB analysis using 28089-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 28089-1-AP (RSAD2 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of A549 using 28089-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 28089-1-AP (RSAD2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse stomach using 28089-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse stomach tissue slide using 28089-1-AP (RSAD2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse stomach using 28089-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse stomach tissue slide using 28089-1-AP (RSAD2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon using 28089-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 28089-1-AP (RSAD2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon using 28089-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 28089-1-AP (RSAD2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: tissu cérébral humain fœtal, cellules A549, tissu de moelle épinière de souris
• Résultats positifs en IHC: tissu d'estomac de souris, tissu de côlon humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 11 publications below
• IHC: See 1 publications below
• IF: See 1 publications below
• IP: See 1 publications below

Informations sur le produit

28089-1-AP cible RSAD2 dans les applications de WB, IP, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: canin, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: RSAD2 Protéine recombinante Ag27733
• Nom complet: radical S-adenosyl methionine domain containing 2
• Masse moléculaire calculée: 361 aa, 42 kDa
• Poids moléculaire observé: 42 kDa
• Numéro d’acquisition GenBank: BC017969
• Symbole du gène: RSAD2
• Identification du gène (NCBI): 91543
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

RSAD2 (radical S-adenosyl methionine domain-containing protein 2), also known as CIG5 (cytomegalovirus-induced gene 5 protein), vig1, viperin or CIG33, displays antiviral effect against HIV-1 virus, hepatitis C virus, human cytomegalovirus, and aphaviruses. Expression of the protein can be induced by interferon.

Publications

Species	Application	Title
human	WB	Int J Mol Sci; Rubella Virus Triggers Type I Interferon Antiviral Response in Cultured Human Neural Cells: Involvement in the Control of Viral Gene Expression and Infectious Progeny Production; Authors - Sayuri Sakuragi
human	IHC	Am J Cancer Res; Identification of potential immune-related circRNA-miRNA-mRNA regulatory network in cutaneous squamous cell carcinoma; Authors - Yuanfeng Wei
human	WB	Cell Mol Biol Lett; 5-Methoxyflavone-induced AMPKα activation inhibits NF-κB and P38 MAPK signaling to attenuate influenza A virus-mediated inflammation and lung injury in vitro and in vivo; Authors - Sushan Yang
mouse	WB	Front Nutr; The Effect of Flammulina velutipes Polysaccharide on Immunization Analyzed by Intestinal Flora and Proteomics.; Authors - Qiongxin Liang
mouse	WB	J Appl Toxicol; Intratracheal instillation of graphene oxide decreases anti-virus responses and lipid contents via suppressing Toll-like receptor 3 in mouse livers.; Authors - Xiaomin Tang
human	WB,IF,IP	Biochemistry; Interactions between Viperin, Vesicle-Associated Membrane Protein A, and Hepatitis C Virus Protein NS5A Modulate Viperin Activity and NS5A Degradation.; Authors - Soumi Ghosh