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Anticorps Polyclonal de lapin anti-RRBP1

RRBP1 Polyclonal Antibody for FC (Intra), IF

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

IF, FC (Intra)

Conjugaison

CoraLite®594 Fluorescent Dye

N° de cat : CL594-22015

Synonymes

DKFZp586A1420, ES/130, ES/130 related protein, ES130, FLJ36146, hES, KIAA1398, Ribosome binding protein 1, Ribosome receptor protein, RRBP1, RRp

Galerie de données de validation

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  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using CoraLite®594-conjugated RRBP1 Polyclonal antibody (CL594-22015)

    IF Staining of HepG2 using CL594-22015

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CoraLite®594-conjugated RRBP1 antibody (CL594-22015) at dilution of 1:200.

  • Flow cytometry (FC) experiment of HeLa cells using CoraLite®594-conjugated RRBP1 Polyclonal antibody (CL594-22015)

    FC experiment of HeLa using CL594-22015

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite®594 Anti-Human RRBP1 (CL594-22015) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Applications testées

Résultats positifs en IFcellules HepG2,
Résultats positifs en cytométriecellules HeLa

Dilution recommandée

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Informations sur le produit

CL594-22015 cible RRBP1 dans les applications de IF, FC (Intra) et montre une réactivité avec des échantillons Humain, souris

Réactivité Humain, souris
Hôte / Isotype Lapin / IgG
Clonalité Polyclonal
Type Anticorps
Immunogène RRBP1 Protéine recombinante Ag16648
Nom complet ribosome binding protein 1 homolog 180kDa (dog)
Masse moléculaire calculée 1410 aa, 152 kDa
Poids moléculaire observé 180 kDa, 109 kDa
Numéro d’acquisition GenBankBC128577
Symbole du gène RRBP1
Identification du gène (NCBI) 6238
Conjugaison CoraLite®594 Fluorescent Dye
Excitation/Emission maxima wavelengths588 nm / 604 nm
Forme Liquide
Méthode de purification Purifié par affinité contre l'antigène
Tampon de stockage PBS avec glycérol à 50 %, Proclin300 à 0,05 % et BSA à 0,5 %, pH 7,3.
Conditions de stockageStocker à -20 °C. Éviter toute exposition à la lumière. L'aliquotage n'est pas nécessaire pour le stockage à -20oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Ribosome-binding protein 1 (RRBP1) is an endoplasmic reticulum membrane protein. It is a 1410 amino acid protein composed of a hydrophobic NH2-terminus that includes a transmembrane domain, a highly conserved tandem repeat (ribosome-binding domain), and an acidic coiled-coil COOH-terminal domain. RRBP1 acts as a ribosome receptor and mediates interaction between the ribosome and the endoplasmic reticulum membrane. It is also involved in early cardiac morphogenesis. (PMID: 22709790; 8812507; 7686155)

Protocole

Product Specific Protocols
IF protocol for CL594 RRBP1 antibody CL594-22015Download protocol
Standard Protocols
Click here to view our Standard Protocols