Anticorps Polyclonal de lapin anti-RRAS

RRAS Polyclonal Antibody for IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IP, IHC, ELISA

Conjugaison

Non conjugué

Publications

3

N° de cat : 27457-1-AP

Synonymes

p23, Ras related protein R Ras, RRAS

---

Galerie de données de validation

• 

  WB analysis using 27457-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 27457-1-AP (RRAS antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A549 using 27457-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 27457-1-AP (RRAS antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of PC-3 using 27457-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 27457-1-AP (RRAS antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HT-29 using 27457-1-AP

  IP result of anti-RRAS(IP:27457-1-AP, 4ug; Detection:27457-1-AP 1:500) with HT-29 cells lysate 3320 ug.

• 

  IHC staining of human renal cell carcinoma using 27457-1-AP

  Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 27457-1-AP (RRAS antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human renal cell carcinoma using 27457-1-AP

  Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 27457-1-AP (RRAS antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse kidney using 27457-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 27457-1-AP (RRAS antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse colon using 27457-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 27457-1-AP (RRAS antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse colon using 27457-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 27457-1-AP (RRAS antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human gliomas using 27457-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 27457-1-AP (RRAS antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human gliomas using 27457-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 27457-1-AP (RRAS antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse kidney using 27457-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 27457-1-AP (RRAS antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

---

Applications testées

• Résultats positifs en WB: cellules A549, cellules HT-29, cellules PC-3
• Résultats positifs en IP: cellules HT-29,
• Résultats positifs en IHC: tissu de carcinome à cellules rénales humain, tissu de côlon de souris, tissu de gliome humain, tissu rénal de souris; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 3 publications below

Informations sur le produit

27457-1-AP cible RRAS dans les applications de WB, IP, IHC, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: RRAS Protéine recombinante Ag26538
• Nom complet: related RAS viral (r-ras) oncogene homolog
• Masse moléculaire calculée: 218 aa, 23 kDa
• Poids moléculaire observé: 23 kDa
• Numéro d’acquisition GenBank: BC016318
• Symbole du gène: RRAS
• Identification du gène (NCBI): 6237
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Publications

Species	Application	Title
human，mouse	WB	Biomaterials; Polymeric nanoparticle mediated inhibition of miR-21 with enhanced miR-124 expression for combinatorial glioblastoma therapy.; Authors - Yuanyuan Liu
human	WB	Transl Cancer Res; Identification of RRAS gene related to nasopharyngeal carcinoma based on pathway and network-based analyses.; Authors - Ruowen Xiao
human	WB	Hepatology; Metabolism-induced tumor activator 1 (MITA1), an Energy Stress-Inducible Long Noncoding RNA, Promotes Hepatocellular Carcinoma Metastasis; Authors - Meilin Ma