Anticorps Monoclonal anti-S6 Ribosomal protein

S6 Ribosomal protein Monoclonal Antibody for FC, IF, WB, ELISA

Hôte / Isotype

Mouse / IgG2a

Réactivité testée

Humain, rat, souris

Applications

WB, IF, FC, ELISA

Conjugaison

Non conjugué

Publications

9

CloneNo.

1C3E10

N° de cat : 66886-1-Ig

Synonymes

40S ribosomal protein S6, Cell and organelle markers, Phosphoprotein NP33, ribosomal protein S6, Ribosome Marker, RPS6

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Galerie de données de validation

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  WB analysis using 66886-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66886-1-Ig (S6 Ribosomal protein antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of NIH/3T3 using 66886-1-Ig

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 66886-1-Ig (S6 Ribosomal protein antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 66886-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66886-1-Ig (S6 Ribosomal protein antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat liver using 66886-1-Ig

  rat liver tissue were subjected to SDS PAGE followed by western blot with 66886-1-Ig (S6 Ribosomal protein antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours.

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  IF Staining of HepG2 using 66886-1-Ig

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using 66886-1-Ig (S6 Ribosomal protein antibody) at dilution of 1:100 and CoraLite488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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  FC experiment of HepG2 using 66886-1-Ig

  1X10^6 HepG2 cells were intracellularly stained with 0.4 ug Anti-Human S6 Ribosomal protein (66886-1-Ig, Clone:1C3E10) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules A431, cellules HEK-293, cellules HSC-T6, cellules Jurkat, cellules NIH/3T3, cellules RAW 264.7, tissu hépatique de rat
• Résultats positifs en IF: cellules HepG2,
• Résultats positifs en cytométrie: cellules HepG2,

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunofluorescence (IF): IF : 1:50-1:500
• Flow Cytometry (FC): FC : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 8 publications below
• IF: See 1 publications below
• FC: See 1 publications below

Informations sur le produit

66886-1-Ig cible S6 Ribosomal protein dans les applications de WB, IF, FC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Mouse / IgG2a
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: S6 Ribosomal protein Protéine recombinante Ag6599
• Nom complet: ribosomal protein S6
• Masse moléculaire calculée: 29 kDa
• Poids moléculaire observé: 29-32 kDa
• Numéro d’acquisition GenBank: BC000524
• Symbole du gène: RPS6
• Identification du gène (NCBI): 6194
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Ribosomal protein S6 (RPS6),Phosphoprotein NP33.It may play an important role in controlling cell growth and proliferation through the selective translation of particular classes of mRNA.Ribosomal protein S6 is the major substrate of protein kinases in eukaryote ribosomes. The phosphorylation is stimulated by growth factors, tumor promoting agents, and mitogens. It is dephosphorylated at growth arrest. Phosphorylated at Ser-235 and Ser-236 by RPS6KA1 and RPS6KA3; phosphorylation at these sites facilitates the assembly of the preinitiation complex.

Publications

Species	Application	Title
human	WB	Genome Biol; A post-transcriptional program of chemoresistance by AU-rich elements and TTP in quiescent leukemic cells.; Authors - Sooncheol Lee
human	WB,FC	Transl Res; Ribosomal proteins as distinct "passengers" of microvesicles: new semantics in myeloma and mesenchymal stem cells' communication.; Authors - Mahmoud Dabbah
human	IF	Microbiol Spectr; Structural Basis and Function of the N Terminus of SARS-CoV-2 Nonstructural Protein 1.; Authors - Kaitao Zhao
mouse	WB	Ecotoxicol Environ Saf; Exposure to DEHP induces testis toxicity and injury through the ROS/mTOR/NLRP3 signaling pathway in immature rats.; Authors - Yifan Hong
human	WB	Sci Rep; YB1 associates with oncogenetic roles and poor prognosis in nasopharyngeal carcinoma.; Authors - Yuting Zhan
rat	WB	Endocrinology; Wnt5a Regulates Junctional Function of Sertoli cells Through PCP-Mediated Effects on mTORC1 and mTORC2.; Authors - Yan Fu

Produits associés

Flow Cytometry Perm Buffer (10X)

PF00011-C