Anticorps Monoclonal anti-RPS3

• Phare
• Validé par KD/KO

RPS3 Monoclonal Antibody for IF, IHC, IP, WB, ELISA

Hôte / Isotype

Mouse / IgG2a

Réactivité testée

Humain, rat, souris

Applications

WB, IP, IHC, IF, FC, ChIP, ELISA

Conjugaison

Non conjugué

Publications

12

CloneNo.

2G7H4

N° de cat : 66046-1-Ig

Synonymes

40S ribosomal protein S3, Cell and organelle markers, ribosomal protein S3, Ribosome Marker, RPS3

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Galerie de données de validation

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  WB analysis using 66046-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66046-1-Ig (RPS3 antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours.

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  WB analysis using 66046-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66046-1-Ig (RPS3 antibody) at dilution of 1:100000 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 66046-1-Ig

  human brain tissue were subjected to SDS PAGE followed by western blot with 66046-1-Ig (RPS3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of Neuro-2a using 66046-1-Ig

  Neuro-2a cells were subjected to SDS PAGE followed by western blot with 66046-1-Ig (RPS3 Antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  WB analysis of RAW 264.7 using 66046-1-Ig

  RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 66046-1-Ig (RPS3 Antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  WB analysis of ROS1728 using 66046-1-Ig

  ROS1728 cells were subjected to SDS PAGE followed by western blot with 66046-1-Ig (RPS3 Antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat brain using 66046-1-Ig

  rat brain tissue were subjected to SDS PAGE followed by western blot with 66046-1-Ig (RPS3 Antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  IP experiment of HEK-293 using 66046-1-Ig

  IP Result of anti-RPS3 (IP:66046-1-Ig, 3ug; Detection:66046-1-Ig 1:2000) with HEK-293 cells lysate 1800ug.

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  IHC staining of human ovary tumor using 66046-1-Ig

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 66046-1-Ig (RPS3 Antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 66046-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 66046-1-Ig(RPS3 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human colon cancer using 66046-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 66046-1-Ig(RPS3 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human pancreas using 66046-1-Ig

  Immunohistochemical analysis of paraffin-embedded human pancreas using 66046-1-Ig(RPS3 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human pancreas using 66046-1-Ig

  Immunohistochemical analysis of paraffin-embedded human pancreas using 66046-1-Ig(RPS3 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human ovary tumor using 66046-1-Ig

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 66046-1-Ig (RPS3 Antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 66046-1-Ig

  Immunofluorescent analysis of (-20℃ Ethanol ) fixed HepG2 cells using 66046-1-Ig(RPS3 antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules HSC-T6, cellules 4T1, cellules HEK-293, cellules HeLa, cellules HepG2, cellules Jurkat, cellules K-562, cellules Neuro-2a, cellules NIH/3T3, cellules RAW 264.7, cellules ROS1728, tissu cérébral de rat, tissu cérébral humain
• Résultats positifs en IP: cellules HEK-293
• Résultats positifs en IHC: tissu de tumeur ovarienne humain, tissu de cancer du côlon humain, tissu pancréatique humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HepG2,

Dilution recommandée

• Western Blot (WB): WB : 1:20000-1:100000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 8 publications below
• IHC: See 2 publications below
• IF: See 2 publications below
• FC: See 1 publications below
• ChIP: See 1 publications below

Informations sur le produit

66046-1-Ig cible RPS3 dans les applications de WB, IP, IHC, IF, FC, ChIP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Mouse / IgG2a
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: RPS3 Protéine recombinante Ag7515
• Nom complet: ribosomal protein S3
• Masse moléculaire calculée: 27 kDa
• Poids moléculaire observé: 33 kDa
• Numéro d’acquisition GenBank: BC003137
• Symbole du gène: RPS3
• Identification du gène (NCBI): 6188
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

40S ribosomal protein S3 (RPS3), also named as SW-cl.26, is a 243 amino acid protein,which contains one KH type-2 domain and belongs to the ribosomal protein S3P family. RPS3 localizes in the cytoplasm. RPS3 is identified in a IGF2BP1-dependent mRNP granule complex, which contains untranslated mRNAs. RPS3 plays a role in repairing various DNA damage acting as a repair UV endonuclease. Nuclear accumulation of RPS3 results in an increase in DNA repair activity to some extent, thereby sustaining neuronal survival. The calculated molecular weight of RPS3 is 26 kDa, but the modified RPS3 is about 33 kDa.

Publications

Species	Application	Title
human	FC	Transl Res; Ribosomal proteins as distinct "passengers" of microvesicles: new semantics in myeloma and mesenchymal stem cells' communication.; Authors - Mahmoud Dabbah
human	IHC	Proc Natl Acad Sci U S A; Local translation in nuclear condensate amyloid bodies.; Authors - Phaedra R Theodoridis
human	WB	PLoS Pathog; Separate domains of G3BP promote efficient clustering of alphavirus replication complexes and recruitment of the translation initiation machinery.; Authors - Benjamin Götte
human	WB	Free Radic Biol Med; Identification of interacting partners of Human Mpv17-like protein with a mitigating effect of mitochondrial dysfunction through mtDNA damage.; Authors - Reiko Iida
human	WB	Oncotarget; Ribosomal protein S3 (rpS3) secreted from various cancer cells is N-linked glycosylated.; Authors - Yong Joong Kim ; KD Validated
human	WB,IF	Front Immunol; Interactome of the Autoimmune Risk Protein ANKRD55.; Authors - Nerea Ugidos