Anticorps Monoclonal anti-RPL3

RPL3 Monoclonal Antibody for IF, IHC, WB,ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

4

CloneNo.

2G1B8

N° de cat : 66130-1-Ig

Synonymes

60S ribosomal protein L3, ribosomal protein L3, RPL3, TARBP B

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Galerie de données de validation

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  WB analysis of RAW 264.7 using 66130-1-Ig

  Western blot analysis of RPL3 in various cell lines using Proteintech antibody 66130-1-Ig at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 66130-1-Ig

  Western blot analysis of RPL3 in various cell lines and tissues using Proteintech antibody 66130-1-Ig at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 66130-1-Ig

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 66130-1-Ig (RPL3 Antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 66130-1-Ig

  Jurkat cells were subjected to SDS PAGE followed by western blot with 66130-1-Ig (RPL3 Antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 66130-1-Ig

  HepG2 cells were subjected to SDS PAGE followed by western blot with 66130-1-Ig (RPL3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 66130-1-Ig

  HeLa cells were subjected to SDS PAGE followed by western blot with 66130-1-Ig (RPL3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 66130-1-Ig

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 66130-1-Ig (RPL3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human liver cancer using 66130-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66130-1-Ig (RPL3 Antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 66130-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66130-1-Ig (RPL3 Antibody) at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 66130-1-Ig

  Immunohistochemical analysis of paraffin-embedded human lung cancer slide using 66130-1-Ig (RPL3 Antibody) at dilution of 1:50.

• 

  IHC staining of human breast cancer using 66130-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66130-1-Ig (RPL3 Antibody) at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 66130-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66130-1-Ig (RPL3 Antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human prostate cancer using 66130-1-Ig

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 66130-1-Ig (RPL3 Antibody) at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human prostate cancer using 66130-1-Ig

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 66130-1-Ig (RPL3 Antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human prostate cancer using 66130-1-Ig

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 66130-1-Ig (RPL3 Antibody) at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human prostate cancer using 66130-1-Ig

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 66130-1-Ig (RPL3 Antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 66130-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66130-1-Ig (RPL3 Antibody) at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 66130-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66130-1-Ig (RPL3 Antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human placenta using 66130-1-Ig

  Immunohistochemical analysis of paraffin-embedded human placenta slide using 66130-1-Ig (RPL3 Antibody) at dilution of 1:50.

• 

  IF Staining of HepG2 using 66130-1-Ig

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using 66130-1-Ig (RPL3 antibody) at dilution of 1:50 and CoraLite488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

• 

  IF Staining of HepG2 using 66130-1-Ig

  Immunofluorescent analysis of HepG2 cells using 66130-1-Ig (RPL3 antibody) at dilution of 1:50 and Rhodamine-Goat anti-Mouse IgG.

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Applications testées

• Résultats positifs en WB: cellules RAW 264.7, cellules HEK-293
• Résultats positifs en IHC: tissu de cancer du foie humain, tissu de cancer de la prostate humain, tissu de cancer du poumon humain, tissu de cancer du sein humain, tissu placentaire humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HepG2,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:8000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 2 publications below
• IF: See 2 publications below

Informations sur le produit

66130-1-Ig cible RPL3 dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: RPL3 Protéine recombinante Ag20400
• Nom complet: ribosomal protein L3
• Masse moléculaire calculée: 46/27 kDa
• Poids moléculaire observé: 46 kDa
• Numéro d’acquisition GenBank: BC012786
• Symbole du gène: RPL3
• Identification du gène (NCBI): 6122
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Ribosomes, the complexes that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. RPL3, also named as TARBP-B and MGC104284, belongs to the ribosomal protein L3P family. This protein is a component of the 60S subunit. It can bind to the HIV-1 TAR mRNA, and it has been suggested that the protein contributes to tat-mediated transactivation. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome.

Publications

Species	Application	Title
human	IF	Nature; ER proteins decipher the tubulin code to regulate organelle distribution; Authors - Pengli Zheng
human	WB	Nat Commun; Structural basis of GTPase-mediated mitochondrial ribosome biogenesis and recycling.; Authors - Hauke S Hillen
human	WB	Elife; METTL18-mediated histidine methylation of RPL3 modulates translation elongation for proteostasis maintenance.; Authors - Eriko Matsuura-Suzuki
mouse	IF	J Biol Chem; Disruption of valosin-containing protein activity causes cardiomyopathy and reveals pleiotropic functions in cardiac homeostasis.; Authors - Matthew J Brody