Anticorps Monoclonal anti-ROR1

• Phare
• Validé par KD/KO

ROR1 Monoclonal Antibody for FC, IHC, WB, ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, souris

Applications

WB, IHC, FC, ELISA

Conjugaison

Non conjugué

Publications

2

CloneNo.

1F5D8

N° de cat : 66923-1-Ig

Synonymes

dJ537F10.1, NTRKR1, ROR1

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Galerie de données de validation

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  WB analysis using 66923-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66923-1-Ig (ROR1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Lamin B1 Monoclonal antibody (HRP-66095) as loading control.

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  WB analysis using 66923-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66923-1-Ig (ROR1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of NIH/3T3 using 66923-1-Ig

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 66923-1-Ig (ROR1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis using 66923-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66923-1-Ig (ROR1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IHC staining of human breast cancer using 66923-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66923-1-Ig (ROR1 antibody) at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 66923-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66923-1-Ig (ROR1 antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  FC experiment of K-562 using 66923-1-Ig

  1X10^6 K-562 cells were intracellularly stained with 0.4 ug Anti-Human ROR1 (66923-1-Ig, Clone:1F5D8) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG1 Isotype Control (MOPC-21) (65124-1-Ig, Clone: MOPC-21) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Applications testées

• Résultats positifs en WB: cellules A549, cellules HEK-293, cellules HeLa, cellules HepG2, cellules K-562, cellules NCI-H1299, cellules NIH/3T3
• Résultats positifs en IHC: tissu de cancer du sein humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en cytométrie: cellules K-562,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:6000
• Immunohistochimie (IHC): IHC : 1:300-1:1200
• Flow Cytometry (FC): FC : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 1 publications below
• IHC: See 2 publications below

Informations sur le produit

66923-1-Ig cible ROR1 dans les applications de WB, IHC, FC, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: ROR1 Protéine recombinante Ag14586
• Nom complet: receptor tyrosine kinase-like orphan receptor 1
• Masse moléculaire calculée: 937 aa, 104 kDa
• Poids moléculaire observé: 130 kDa
• Numéro d’acquisition GenBank: BC006374
• Symbole du gène: ROR1
• Identification du gène (NCBI): 4919
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

ROR1 is a member of the RTK family of orphan receptors related to muscle-specific kinase and Trk neurotrophin receptors (PMID: 18546292). ROR1 is primarily expressed by neural crest cells during embryogenesis. High expression of ROR1 is reported in many types of malignancies and is thought to be involved in tumor growth, apoptosis, and epithelial-mesenchymal transition (PMID: 26245996). The human ROR1 gene encodes a 937 amino acid protein with an Ig-like domain, a cysteine-rich domain, a kringle domain, a tyrosine kinase domain, and a proline-rich domain. The calculated molecular weight of ROR1 is 104 kDa, but ROR1 has multiple N-glycosylation sites that generate post-translationally modified ROR1 at 130 kDa (PMID: 24752542).

Publications

Species	Application	Title
human	WB,IHC	Acta Biochim Biophys Sin (Shanghai); SETD8 induces stemness and epithelial-mesenchymal transition of pancreatic cancer cells by regulating ROR1 expression.; Authors - Mengqi Liu ; KD Validated
human	IHC	Int Immunopharmacol; ROR1/STAT3 positive feedback loop facilitates cartilage degeneration in Osteoarthritis through activation of NF-κB signaling pathway; Authors - Wei Huang

Produits associés

Flow Cytometry Perm Buffer (10X)

PF00011-C