Anticorps Monoclonal anti-ROCK1

ROCK1 Monoclonal Antibody for WB,ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, rat, souris

Applications

WB, ELISA

Conjugaison

Non conjugué

CloneNo.

3G5E12

N° de cat : 66782-1-Ig

Synonymes

p160 ROCK 1, P160ROCK, PRO0435, ROCK1



Applications testées

Résultats positifs en WBcellules HeLa, cellules HEK-293, cellules HepG2, cellules HSC-T6, cellules Jurkat, cellules NIH/3T3, cellules RAW 264.7

Dilution recommandée

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Applications publiées

WBSee 3 publications below

Informations sur le produit

66782-1-Ig cible ROCK1 dans les applications de WB, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

Réactivité Humain, rat, souris
Réactivité citéeHumain, souris
Hôte / Isotype Mouse / IgG1
Clonalité Monoclonal
Type Anticorps
Immunogène ROCK1 Protéine recombinante Ag16317
Nom complet Rho-associated, coiled-coil containing protein kinase 1
Masse moléculaire calculée 1354 aa, 158 kDa
Poids moléculaire observé 150-160 kDa
Numéro d’acquisition GenBankBC113114
Symbole du gène ROCK1
Identification du gène (NCBI) 6093
Conjugaison Non conjugué
Forme Liquide
Méthode de purification Purification par protéine G
Tampon de stockage PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
Conditions de stockageStocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20oC Les 20ul contiennent 0,1% de BSA.

Informations générales

ROCK1, also named as p160ROCK and NY-REN-35, belongs to the protein kinase superfamily and AGC Ser/Thr protein kinase family. ROCK1 phosphorylates and activates DAPK3, which then regulates myosin light chain phosphatase through phosphorylation of MYPT1 thereby regulating the assembly of the actin cytoskeleton, cell migration, invasiveness of tumor cells, smooth muscle contraction and neurite outgrowth. ROCK1 is required for centromere positioning and centromere-dependent exit from mitosis. It is necessary for apoptotic membrane blebbing. ROCK1 catalyzes the reaction: ATP + a protein = ADP + a phosphoprotein.

Protocole

Product Specific Protocols
WB protocol for ROCK1 antibody 66782-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

J Cell Biochem

Lnc-MUC20-9 binds to ROCK1 and functions as a tumor suppressor in bladder cancer.

Authors - Ranran Dai
mouseWB

Am J Transl Res

β-catenin regulates myocardial ischemia/reperfusion injury following heterotopic heart transplantation in mice by modulating PTEN pathways.

Authors - Qian Ban
mouseWB

Food Funct

Strains producing different short-chain fatty acids alleviate DSS-induced ulcerative colitis by regulating intestinal microecology

Authors - Shuo Yang

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Balawant (Verified Customer) (12-20-2022)

I have used this antibody for western blot and it is working great

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:700
  • Cell Tissue Type: renal Cancer cell line (cake-2,caki-2)