Anticorps Monoclonal anti-ROCK1
ROCK1 Monoclonal Antibody for WB,ELISA
Hôte / Isotype
Mouse / IgG1
Réactivité testée
Humain, rat, souris
Applications
WB, ELISA
Conjugaison
Non conjugué
CloneNo.
3G5E12
N° de cat : 66782-1-Ig
Synonymes
Galerie de données de validation
Applications testées
Résultats positifs en WB | cellules HeLa, cellules HEK-293, cellules HepG2, cellules HSC-T6, cellules Jurkat, cellules NIH/3T3, cellules RAW 264.7 |
Dilution recommandée
Application | Dilution |
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Western Blot (WB) | WB : 1:1000-1:4000 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, check data in validation data gallery |
Applications publiées
WB | See 3 publications below |
Informations sur le produit
66782-1-Ig cible ROCK1 dans les applications de WB, ELISA et montre une réactivité avec des échantillons Humain, rat, souris
Réactivité | Humain, rat, souris |
Réactivité citée | Humain, souris |
Hôte / Isotype | Mouse / IgG1 |
Clonalité | Monoclonal |
Type | Anticorps |
Immunogène | ROCK1 Protéine recombinante Ag16317 |
Nom complet | Rho-associated, coiled-coil containing protein kinase 1 |
Masse moléculaire calculée | 1354 aa, 158 kDa |
Poids moléculaire observé | 150-160 kDa |
Numéro d’acquisition GenBank | BC113114 |
Symbole du gène | ROCK1 |
Identification du gène (NCBI) | 6093 |
Conjugaison | Non conjugué |
Forme | Liquide |
Méthode de purification | Purification par protéine G |
Tampon de stockage | PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3 |
Conditions de stockage | Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20oC Les 20ul contiennent 0,1% de BSA. |
Informations générales
ROCK1, also named as p160ROCK and NY-REN-35, belongs to the protein kinase superfamily and AGC Ser/Thr protein kinase family. ROCK1 phosphorylates and activates DAPK3, which then regulates myosin light chain phosphatase through phosphorylation of MYPT1 thereby regulating the assembly of the actin cytoskeleton, cell migration, invasiveness of tumor cells, smooth muscle contraction and neurite outgrowth. ROCK1 is required for centromere positioning and centromere-dependent exit from mitosis. It is necessary for apoptotic membrane blebbing. ROCK1 catalyzes the reaction: ATP + a protein = ADP + a phosphoprotein.
Protocole
Product Specific Protocols | |
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WB protocol for ROCK1 antibody 66782-1-Ig | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
J Cell Biochem Lnc-MUC20-9 binds to ROCK1 and functions as a tumor suppressor in bladder cancer. | ||
Am J Transl Res β-catenin regulates myocardial ischemia/reperfusion injury following heterotopic heart transplantation in mice by modulating PTEN pathways. | ||
Food Funct Strains producing different short-chain fatty acids alleviate DSS-induced ulcerative colitis by regulating intestinal microecology |
Avis
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH Balawant (Verified Customer) (12-20-2022) | I have used this antibody for western blot and it is working great
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