Anticorps Monoclonal anti-RHEB

RHEB Monoclonal Antibody for IF, WB, ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, souris

Applications

WB, IF, ELISA

Conjugaison

Non conjugué

CloneNo.

2F9H8

N° de cat : 66714-1-Ig

Synonymes

GTP binding protein Rheb, Ras homolog enriched in brain, RHEB, RHEB2

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Galerie de données de validation

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  WB analysis of fetal human brain using 66714-1-Ig

  fetal human brain tissue were subjected to SDS PAGE followed by western blot with 66714-1-Ig (RHEB antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 66714-1-Ig

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 66714-1-Ig (RHEB antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 66714-1-Ig

  HeLa cells were subjected to SDS PAGE followed by western blot with 66714-1-Ig (RHEB antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of DU 145 using 66714-1-Ig

  DU 145 cells were subjected to SDS PAGE followed by western blot with 66714-1-Ig (RHEB antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IF Staining of human pancreas cancer using 66714-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed human pancreas cancer tissue using RHEB antibody (66714-1-Ig, Clone: 2F9H8 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

• 

  IF Staining of mouse pancreas using 66714-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed mouse pancreas tissue using RHEB antibody (66714-1-Ig, Clone: 2F9H8 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

• 

  IF Staining of human pancreas cancer using 66714-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed human pancreas cancer tissue using RHEB antibody (66714-1-Ig, Clone: 2F9H8 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

• 

  IF Staining of mouse pancreas using 66714-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed mouse pancreas tissue using RHEB antibody (66714-1-Ig, Clone: 2F9H8 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Applications testées

• Résultats positifs en WB: tissu cérébral humain fœtal, cellules DU 145, cellules HeLa, tissu cérébral de souris
• Résultats positifs en IF: tissu de cancer du pancréas humain, tissu pancréatique de souris

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:3000
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

66714-1-Ig cible RHEB dans les applications de WB, IF, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: RHEB Protéine recombinante Ag8887
• Nom complet: Ras homolog enriched in brain
• Masse moléculaire calculée: 184 aa, 20 kDa
• Poids moléculaire observé: 18 kDa
• Numéro d’acquisition GenBank: BC016155
• Symbole du gène: RHEB
• Identification du gène (NCBI): 6009
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Rheb is a member of the Ras superfamily small GTPases that plays important roles in the regulation of protein synthesis and growth in response to nutrient and growth factors. It is well known to bind and activate mTOR, a serine/threonine kinase which augments protein synthesis. Rheb has GTPase activity and shuttles between a GDP-bound form and a GTP-bound form, and farnesylation of the protein is required for this activity.