Anticorps Monoclonal anti-RGS4

RGS4 Monoclonal Antibody for WB,ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, porc, rat, souris

Applications

WB, IHC,ELISA

Conjugaison

Non conjugué

Publications

1

CloneNo.

1E2E2

N° de cat : 66441-1-Ig

Synonymes

RGP4, RGS4, SCZD9

---

Galerie de données de validation

• 

  WB analysis of Neuro-2a using 66441-1-Ig

  Neuro-2a cells were subjected to SDS PAGE followed by western blot with 66441-1-Ig (RGS4 Antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of C6 using 66441-1-Ig

  C6 cells were subjected to SDS PAGE followed by western blot with 66441-1-Ig (RGS4 Antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Y79 using 66441-1-Ig

  Y79 cells were subjected to SDS PAGE followed by western blot with 66441-1-Ig (RGS4 Antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of SH-SY5Y using 66441-1-Ig

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 66441-1-Ig (RGS4 Antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of U-251 using 66441-1-Ig

  U-251 cells were subjected to SDS PAGE followed by western blot with 66441-1-Ig (RGS4 Antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Raji using 66441-1-Ig

  Raji cells were subjected to SDS PAGE followed by western blot with 66441-1-Ig (RGS4 Antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

---

Applications testées

• Résultats positifs en WB: cellules Neuro-2a, cellules C6, cellules Raji, cellules SH-SY5Y, cellules U-251, cellules Y79

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:12000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• IHC: See 1 publications below

Informations sur le produit

66441-1-Ig cible RGS4 dans les applications de WB, IHC,ELISA et montre une réactivité avec des échantillons Humain, porc, rat, souris

• Réactivité: Humain, porc, rat, souris
• Réactivité citée: souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: RGS4 Protéine recombinante Ag6029
• Nom complet: regulator of G-protein signaling 4
• Masse moléculaire calculée: 23 kDa
• Poids moléculaire observé: 34 kDa
• Numéro d’acquisition GenBank: BC051869
• Symbole du gène: RGS4
• Identification du gène (NCBI): 5999
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Regulator of G protein signaling (RGS) proteins are a large family of highly diverse, multifunctional signaling proteins. RGS4 is one of seven members of a classic R4 RGS protein that accelerates the GTPase activity of the Gai/o and Gaq/11 family members. RGS4 is highly expressed in multiple brain regions including the cerebral cortex, amygdala, hippocampus, and striatum. Extensive studies have demonstrated that RGS4 plays an important role in regulating smooth muscle contraction, cardiomyocyte development, neural plasticity and psychiatric disorders.

Publications

Species	Application	Title
mouse	IHC	Hippocampus; NRSF deficiency leads to abnormal postnatal development of dentate gyrus and impairment of progenitors in subgranular zone of hippocampus.; Authors - Yan-Cong Wang