Anticorps Polyclonal de lapin anti-RCOR1

RCOR1 Polyclonal Antibody for WB,ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, ELISA

Conjugaison

Non conjugué

Publications

5

N° de cat : 27686-1-AP

Synonymes

COREST, KIAA0071, Protein CoREST, RCOR, RCOR1, RCOR1Specific, REST corepressor 1

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Galerie de données de validation

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  WB analysis of C2C12 using 27686-1-AP

  C2C12 cell were subjected to SDS PAGE followed by western blot with 27686-1-AP (RCOR1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of PC-12 using 27686-1-AP

  PC-12 cells were subjected to SDS PAGE followed by western blot with 27686-1-AP (RCOR1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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Applications testées

• Résultats positifs en WB: cellule C2C12, cellules PC-12

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 4 publications below
• IHC: See 1 publications below

Informations sur le produit

27686-1-AP cible RCOR1 dans les applications de WB, IHC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: RCOR1 Protéine recombinante Ag26770
• Nom complet: REST corepressor 1
• Masse moléculaire calculée: 53 kDa
• Poids moléculaire observé: 60-65 kDa
• Numéro d’acquisition GenBank: NM_015156
• Symbole du gène: RCOR1
• Identification du gène (NCBI): 23186
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

RCOR1, also named as REST corepressor 1, is a 485 amino acid protein, which belongs to the CoREST family. RCOR1 is a essential component of the BHC complex, a corepressor complex that represses transcription of neuron-specific genes in non-neuronal cells. The BHC complex is recruited at RE1/NRSE sites by REST and acts by deacetylating and demethylating specific sites on histones, thereby acting as a chromatin modifier. The calculated molecular weight of RCOR1 is a 53 kDa, but the phosphorylated RCOR1 is about 60-65 kDa.

Publications

Species	Application	Title
human	WB	EMBO J; The chromatin remodeler SRCAP promotes self-renewal of intestinal stem cells.; Authors - Buqing Ye
rat	WB	Biochem Biophys Res Commun; Increased NRSF/REST in anterior cingulate cortex contributes to diabetes-related neuropathic pain.; Authors - Xu Xiao-Die
human	IHC	Ann Diagn Pathol; microRNA 155 up regulation in the CNS is strongly correlated to Down's syndrome dementia.; Authors - Esmerina Tili
human	WB	Nat Commun; Demethylase-independent roles of LSD1 in regulating enhancers and cell fate transition; Authors - Cheng Zeng ; KO Validated
human	WB	Cell Chem Biol; HDAC3 and HDAC8 PROTAC dual degrader reveals roles of histone acetylation in gene regulation; Authors - Yufeng Xiao