Anticorps Monoclonal anti-RCAS1

RCAS1 Monoclonal Antibody for FC, IF, IHC, WB, ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF, FC, ELISA

Conjugaison

Non conjugué

Publications

1

CloneNo.

4H8A12

N° de cat : 66170-1-Ig

Synonymes

EB9, EBAG9, PDAF, RCAS1

---

Galerie de données de validation

• 

  WB analysis of HEK-293 using 66170-1-Ig

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 66170-1-Ig (RCAS1 antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human breast cancer using 66170-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66170-1-Ig (RCAS1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 66170-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66170-1-Ig (RCAS1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 66170-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66170-1-Ig (RCAS1 antibody at dilution of 1:50. Heat mediated antigen retrieved with Citric acid buffer, pH6.0.

• 

  IHC staining of human breast cancer using 66170-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66170-1-Ig (RCAS1 antibody at dilution of 1:50. Heat mediated antigen retrieved with Citric acid buffer, pH6.0.

• 

  IHC staining of human lung cancer using 66170-1-Ig

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66170-1-Ig (RCAS1 antibody at dilution of 1:50. Heat mediated antigen retrieved with Citric acid buffer, pH6.0.

• 

  IHC staining of human lung cancer using 66170-1-Ig

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66170-1-Ig (RCAS1 antibody at dilution of 1:50. Heat mediated antigen retrieved with Citric acid buffer, pH6.0.

• 

  IF Staining of human breast cancer using 66170-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed human breast cancer tissue using RCAS1 antibody (66170-1-Ig, Clone: 4H8A12 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

• 

  IF Staining of MCF-7 using 66170-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using RCAS1 antibody (66170-1-Ig, Clone: 4H8A12 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-phalloidin (red).

• 

  FC experiment of Jurkat using 66170-1-Ig

  1X10^6 Jurkat cells were intracellularly stained with 0.2 ug Anti-Human RCAS1 (66170-1-Ig, Clone:4H8A12) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (green), and 0.2 ug Mouse IgG1 Isotype Control (66360-1-Ig, Clone: T1F8D3F10) (black). Cells were fixed with 90% MeOH.

---

Applications testées

• Résultats positifs en WB: cellules HEK-293
• Résultats positifs en IHC: tissu de cancer du sein humain, tissu de cancer du poumon humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules MCF-7, tissu de cancer du sein humain
• Résultats positifs en cytométrie: cellules Jurkat,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:200-1:800
• Flow Cytometry (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 1 publications below
• IF: See 1 publications below

Informations sur le produit

66170-1-Ig cible RCAS1 dans les applications de WB, IHC, IF, FC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: RCAS1 Protéine recombinante Ag2905
• Nom complet: estrogen receptor binding site associated, antigen, 9
• Masse moléculaire calculée: 213 aa, 24 kDa
• Poids moléculaire observé: 34 kDa
• Numéro d’acquisition GenBank: BC017729
• Symbole du gène: EBAG9
• Identification du gène (NCBI): 9166
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Estrogen receptor-binding fragment-associated antigen 9 (EBAG9) gene was identified as an estrogen-responsive gene. The gene product, receptor-binding cancer antigen expressed on SiSo cells (RCAS1), is associated with aggressive characteristics and poor overall survival for 15 different human malignancies. The correlation between RCAS1 expression and several clinicopathological variables, including tumor size, clinical stage, invasion depth and lymph node metastasis highlights this molecule's clinical significance. Expression of RCAS1 in tumor cells plays an important role in evasion from host immune system resulting tumor progression, invasion and metastasis. Further exploration of RCAS1 biological function will facilitate development of novel therapeutic strategies that target RCAS1.

Publications

Species	Application	Title
mouse	WB,IF	Mol Med Rep; RCAS1 increases cell morphological changes in murine fibroblasts by reducing p38 phosphorylation; Authors - Takuya Nishinakagawa