Anticorps Monoclonal anti-RALBP1

RALBP1 Monoclonal Antibody for FC, WB,ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, souris

Applications

WB, FC,ELISA

Conjugaison

Non conjugué

CloneNo.

1B2F5

N° de cat : 67359-1-Ig

Synonymes

76 kDa Ral interacting protein, DNP SG ATPase, Ral interacting protein 1, ralA binding protein 1, RALBP1, RIP1, RLIP1, RLIP76

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Galerie de données de validation

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  WB analysis of NIH/3T3 using 67359-1-Ig

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 67359-1-Ig (RALBP1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 67359-1-Ig

  Jurkat cells were subjected to SDS PAGE followed by western blot with 67359-1-Ig (RALBP1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 67359-1-Ig

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 67359-1-Ig (RALBP1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of LNCaP using 67359-1-Ig

  LNCaP cells were subjected to SDS PAGE followed by western blot with 67359-1-Ig (RALBP1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 67359-1-Ig

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 67359-1-Ig (RALBP1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  FC experiment of HEK-293 using 67359-1-Ig

  1X10^6 HEK-293 cells were stained with 0.20 ug/test Anti-Human RALBP1 (67359-1-Ig, Clone:1B2F5) (red) or 0.20 ug control antibody (blue) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) with dilution 1:1000. Fixed with90% MeOH.

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Applications testées

• Résultats positifs en WB: cellules NIH/3T3, cellules HEK-293, cellules Jurkat, cellules LNCaP, cellules MCF-7
• Résultats positifs en cytométrie: cellules HEK-293

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:6000
• Flow Cytometry (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

67359-1-Ig cible RALBP1 dans les applications de WB, FC,ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: RALBP1 Protéine recombinante Ag28618
• Nom complet: ralA binding protein 1
• Masse moléculaire calculée: 655 aa, 76 kDa
• Poids moléculaire observé: 95 kDa
• Numéro d’acquisition GenBank: BC013126
• Symbole du gène: RALBP1
• Identification du gène (NCBI): 10928
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20 ℃. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.