Anticorps Polyclonal de lapin anti-RAD23A

RAD23A Polyclonal Antibody for IHC, IP, WB,ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (1)

Applications

WB, IP, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

3

N° de cat : 51033-1-AP

Synonymes

HHR23A, HR23A, RAD23A

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Galerie de données de validation

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  WB analysis of mouse spleen using 51033-1-AP

  mouse spleen tissue were subjected to SDS PAGE followed by western blot with 51033-1-AP (RAD23A Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of rat spleen using 51033-1-AP

  rat spleen tissue were subjected to SDS PAGE followed by western blot with 51033-1-AP (RAD23A Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 51033-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 51033-1-AP (RAD23A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of Jurkat using 51033-1-AP

  IP Result of anti-RAD23A (IP:51033-1-AP, 4ug; Detection:51033-1-AP 1:500) with Jurkat cells lysate 4000ug.

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  IHC staining of human lymphoma using 51033-1-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma using 51033-1-AP (RAD23A antibody) at dilution of 1:50 (under 10x lens).

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Applications testées

• Résultats positifs en WB: tissu splénique de souris, cellules Jurkat, tissu splénique de rat
• Résultats positifs en IP: cellules Jurkat
• Résultats positifs en IHC: tissu de lymphome humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 3 publications below
• IF: See 1 publications below

Informations sur le produit

51033-1-AP cible RAD23A dans les applications de WB, IP, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, singe, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: RAD23A Protéine recombinante Ag0716
• Nom complet: RAD23 homolog A (S. cerevisiae)
• Masse moléculaire calculée: 40 kDa
• Poids moléculaire observé: 55 kDa
• Numéro d’acquisition GenBank: BC014026
• Symbole du gène: RAD23A
• Identification du gène (NCBI): 5886
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20 ℃. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

RAD23A, also named as HHR23A, belongs to the RAD23 family. It is a multiubiquitin chain receptor involved in modulation of proteasomal degradation. RAD23A binds to 'Lys-48'-linked polyubiquitin chains in a length-dependent manner and with a lower affinity to 'Lys-63'-linked polyubiquitin chains. It is proposed to be capable to bind simultaneously to the 26S proteasome and to polyubiquitinated substrates and to deliver ubiquitinated proteins to the proteasome. It is involved in nucleotide excision repair and is thought to be functional equivalent for RAD23B in global genome nucleotide excision repair (GG-NER) by association with XPC. In vitro, the XPC:RAD23A dimer has NER activity. Can stabilize XPC. It is also involved in vpr-dependent replication of HIV-1 in non-proliferating cells and primary macrophages and is required for the association of HIV-1 vpr with the host proteasome. This antibody is a rabbit polyclonal antibody raised against a fusion protein of full length human RAD23A.

Publications

Species	Application	Title
human	WB	Front Immunol; PDLIM7 Synergizes With PDLIM2 and p62/Sqstm1 to Inhibit Inflammatory Signaling by Promoting Degradation of the p65 Subunit of NF-κB.; Authors - Aya Jodo
monkey	WB, IF	PLoS One; Valosin-containing protein (VCP/p97) is an activator of wild-type ataxin-3.; Authors - Laço Mário N MN
mouse	WB	Cell Rep; Autism-linked UBE3A gain-of-function mutation causes interneuron and behavioral phenotypes when inherited maternally or paternally in mice; Authors - Lei Xing