Anticorps Monoclonal anti-RAC2

• Phare
• Validé par KD/KO

RAC2 Monoclonal Antibody for IHC, WB, ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF, coIP, ELISA

Conjugaison

Non conjugué

Publications

11

CloneNo.

4C2H6

N° de cat : 60077-1-Ig

Synonymes

EN 7, Gx, HSPC022, p21 Rac2, RAC2, Small G protein

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Galerie de données de validation

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  WB analysis using 60077-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 60077-1-Ig (RAC2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  IHC staining of human tonsillitis using 60077-1-Ig

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 60077-1-Ig (RAC2 antibody) at dilution of 1:8000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human tonsillitis using 60077-1-Ig

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 60077-1-Ig (RAC2 antibody) at dilution of 1:8000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human tonsillitis using 60077-1-Ig

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 60077-1-Ig (RAC2 antibody) at dilution of 1:8000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human tonsillitis using 60077-1-Ig

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 60077-1-Ig (RAC2 antibody) at dilution of 1:8000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: cellules Jurkat, cellules HSC-T6, cellules MCF-7, cellules NIH/3T3, cellules RAW 264.7
• Résultats positifs en IHC: tissu d'amygdalite humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:20000
• Immunohistochimie (IHC): IHC : 1:4000-1:16000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 3 publications below
• WB: See 11 publications below
• IHC: See 2 publications below
• IF: See 1 publications below
• CoIP: See 1 publications below

Informations sur le produit

60077-1-Ig cible RAC2 dans les applications de WB, IHC, IF, coIP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: RAC2 Protéine recombinante Ag1202
• Nom complet: ras-related C3 botulinum toxin substrate 2 (rho family, small GTP binding protein Rac2)
• Masse moléculaire calculée: 21 kDa
• Poids moléculaire observé: 21 kDa
• Numéro d’acquisition GenBank: BC001485
• Symbole du gène: RAC2
• Identification du gène (NCBI): 5880
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

RAC2, also named as GX, belongs to the small GTPase superfamily and Rho family. RAC2 is a plasma membrane-associated small GTPase which cycles between an active GTP-bound and inactive GDP-bound state. In active state binds to a variety of effector proteins to regulate cellular responses, such as secretory processes, phagocytose of apoptotic cells and epithelial cell polarization. RAC2 augments the production of reactive oxygen species (ROS) by NADPH oxidase. Defects in RAC2 are the cause of neutrophil immunodeficiency syndrome.

Publications

Species	Application	Title
rat	WB,coIP	ACS Nano; Oxidation of Reduced Graphene Oxide via Cellular Redox Signaling Modulates Actin-Mediated Neurotransmission.; Authors - Yiyuan Kang
mouse	WB	J Exp Med; Chemokine-coupled β2 integrin-induced macrophage Rac2-Myosin IIA interaction regulates VEGF-A mRNA stability and arteriogenesis.; Authors - Alan R Morrison ; KO Validated
mouse	WB	Prog Neurobiol; A γ-adducin cleavage fragment induces neurite deficits and synaptic dysfunction in Alzheimer's disease.; Authors - Min Xiong
human	WB,IF	Cell Mol Immunol; DOCK2 regulates antifungal immunity by regulating RAC GTPase activity.; Authors - Xiaojian Ma
mouse	WB	Sci Signal; Rac signal adaptation controls neutrophil mobilization from the bone marrow.; Authors - Carlo Cosimo Campa
human	WB	Oncotarget; Upregulated long non-coding RNA AFAP1-AS1 expression is associated with progression and poor prognosis of nasopharyngeal carcinoma.; Authors - Hao Bo