Anticorps Polyclonal de lapin anti-RAB34

• Phare
• Validé par KD/KO

RAB34 Polyclonal Antibody for WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain et plus (1)

Applications

WB, IF, ELISA

Conjugaison

Non conjugué

Publications

6

N° de cat : 27435-1-AP

Synonymes

RAB34, RAB39, RAH, Ras related protein Rab 34, Ras related protein Rab 39, Ras related protein Rah

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Galerie de données de validation

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  WB analysis using 27435-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 27435-1-AP (RAB34 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 27435-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 27435-1-AP (RAB34 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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Applications testées

• Résultats positifs en WB: cellules A431, cellules A549, cellules HeLa, cellules U2OS

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 3 publications below
• IF: See 5 publications below

Informations sur le produit

27435-1-AP cible RAB34 dans les applications de WB, IF, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: RAB34 Protéine recombinante Ag26696
• Nom complet: RAB34, member RAS oncogene family
• Masse moléculaire calculée: 251 aa, 28 kDa
• Poids moléculaire observé: 29 kDa
• Numéro d’acquisition GenBank: BC016841
• Symbole du gène: RAB34
• Identification du gène (NCBI): 83871
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

RAB34, a Rab protein family member, is involved in repositioning of lysosomes, activation of micropinocytosis, and protein transport. RAB34 is aberrantly expressed in various cancers and exhibits oncogenic properties. Recently, Rab34 is identified as a ciliary protein. Rab34 is required for internal assembly of cilia but not for cilia built on the cell surface.

Publications

Species	Application	Title
mouse	IF, WB	Curr Biol; Rab34 GTPase mediates ciliary membrane formation in the intracellular ciliogenesis pathway.; Authors - Anil Kumar Ganga ; KD Validated
human	IF	Elife; Centriolar satellites expedite mother centriole remodeling to promote ciliogenesis; Authors - Emma A Hall
human	WB,IF	Biomedicines; Signaling Switching from Hedgehog-GLI to MAPK Signaling Potentially Serves as a Compensatory Mechanism in Melanoma Cell Lines Resistant to GANT-61; Authors - Nikolina Piteša
human	WB	Hum Mol Genet; Pathogenic RAB34 variants impair primary cilium assembly and cause a novel oral-facial-digital syndrome; Authors - Ange-Line Bruel
mouse	IF	bioRxiv; A hierarchical pathway for assembly of the distal appendages that organize primary cilia; Authors - Tomoharu Kanie
mouse	IF	bioRxiv; Myristoylated Neuronal Calcium Sensor-1 captures the ciliary vesicle at distal appendages; Authors - Tomoharu Kanie