Anticorps Monoclonal anti-RAB14

RAB14 Monoclonal Antibody for IHC, WB, ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, porc, rat, souris

Applications

WB, IHC, ELISA

Conjugaison

Non conjugué

CloneNo.

3G7B3

N° de cat : 67953-1-Ig

Synonymes

FBP, RAB 14, RAB14, Ras related protein Rab 14

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Galerie de données de validation

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  WB analysis using 67953-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67953-1-Ig (RAB14 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control.

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  WB analysis of pig brain using 67953-1-Ig

  pig brain tissue were subjected to SDS PAGE followed by western blot with 67953-1-Ig (RAB14 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of pig brain using 67953-1-Ig

  pig brain tissue were subjected to SDS PAGE followed by western blot with 67953-1-Ig (RAB14 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of HCT 116 using 67953-1-Ig

  HCT 116 cells were subjected to SDS PAGE followed by western blot with 67953-1-Ig (RAB14 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of K-562 using 67953-1-Ig

  K-562 cells were subjected to SDS PAGE followed by western blot with 67953-1-Ig (RAB14 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of HSC-T6 using 67953-1-Ig

  HSC-T6 cells were subjected to SDS PAGE followed by western blot with 67953-1-Ig (RAB14 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of NIH/3T3 using 67953-1-Ig

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 67953-1-Ig (RAB14 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse ovary using 67953-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse ovary tissue slide using 67953-1-Ig (RAB14 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 67953-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67953-1-Ig (RAB14 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse ovary using 67953-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse ovary tissue slide using 67953-1-Ig (RAB14 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse ovary using 67953-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse ovary tissue slide using 67953-1-Ig (RAB14 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: cellules LNCaP, cellules HCT 116, cellules HEK-293, cellules HeLa, cellules HSC-T6, cellules Jurkat, cellules K-562, cellules NIH/3T3, tissu cérébral de lapin, tissu cérébral de porc, tissu cérébral de rat, tissu cérébral de souris
• Résultats positifs en IHC: tissu ovarien de souris, tissu de cancer du côlon humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochimie (IHC): IHC : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

67953-1-Ig cible RAB14 dans les applications de WB, IHC, ELISA et montre une réactivité avec des échantillons Humain, porc, rat, souris

• Réactivité: Humain, porc, rat, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: RAB14 Protéine recombinante Ag8167
• Nom complet: RAB14, member RAS oncogene family
• Masse moléculaire calculée: 215 aa, 24 kDa
• Numéro d’acquisition GenBank: BC006081
• Symbole du gène: RAB14
• Identification du gène (NCBI): 51552
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.