Anticorps Monoclonal anti-Prohibitin 2

• Phare
• Validé par KD/KO

Prohibitin 2 Monoclonal Antibody for IF, IHC, WB, ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

11

CloneNo.

1D9C7

N° de cat : 66424-1-Ig

Synonymes

BAP, Bap37, D prohibitin, p22, PHB2, PNAS 141, prohibitin 2, REA

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Galerie de données de validation

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  WB analysis using 66424-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66424-1-Ig (Prohibitin 2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Beta Actin Monoclonal antibody (HRP-66009) as loading control.

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  WB analysis of HeLa using 66424-1-Ig

  WB result of Prohibitin 2 antibody (66424-1-Ig; 1:40000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Prohibitin 2 transfected HeLa cells.

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  WB analysis using 66424-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66424-1-Ig (Prohibitin 2 antibody) at dilution of 1:40000 incubated at room temperature for 1.5 hours.

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  IHC staining of human breast cancer using 66424-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66424-1-Ig (Prohibitin 2 antibody at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 66424-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66424-1-Ig (Prohibitin 2 antibody at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human heart using 66424-1-Ig

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 66424-1-Ig (Prohibitin 2 antibody at dilution of 1:400 (under 10x lens).

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  IHC staining of human heart using 66424-1-Ig

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 66424-1-Ig (Prohibitin 2 antibody at dilution of 1:400 (under 40x lens).

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  IF Staining of HepG2 using 66424-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 66424-1-Ig (Prohibitin 2 antibody) at dilution of 1:100 and CoraLite488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). F-actin was labelled in red with Phalloidin.

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Applications testées

• Résultats positifs en WB: cellules Jurkat, cellules 4T1, cellules HEK-293, cellules HeLa, cellules HSC-T6, cellules LNCaP, cellules MCF-7, cellules NIH/3T3, cellules ROS1728
• Résultats positifs en IHC: tissu de cancer du sein humain, tissu cardiaque humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HepG2,

Dilution recommandée

• Western Blot (WB): WB : 1:10000-1:50000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 4 publications below
• WB: See 8 publications below
• IHC: See 1 publications below
• IF: See 6 publications below

Informations sur le produit

66424-1-Ig cible Prohibitin 2 dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: Prohibitin 2 Protéine recombinante Ag2977
• Nom complet: prohibitin 2
• Masse moléculaire calculée: 299 aa, 33 kDa
• Poids moléculaire observé: 33 kDa
• Numéro d’acquisition GenBank: BC014766
• Symbole du gène: PHB2
• Identification du gène (NCBI): 11331
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

PHB2, also named as BAP, REA, D-prohibitin, BAP37 and Prohibitin-2, belongs to the prohibitin family. It acts as a mediator of transcriptional repression by nuclear hormone receptors via recruitment of histone deacetylases. PHB2 functions as an estrogen receptor (ER)-selective coregulator that potentiates the inhibitory activities of antiestrogens and represses the activity of estrogens. It competes with NCOA1 for modulation of ER transcriptional activity. PHB2 is probably involved in regulating mitochondrial respiration activity and in aging.(PMID: 10359819)

Publications

Species	Application	Title
human	WB	EMBO Rep; NDP52 interacts with mitochondrial RNA poly(A) polymerase to promote mitophagy.; Authors - Norihiko Furuya
human	WB	Elife; Genome-wide CRISPRi screening identifies OCIAD1 as a prohibitin client and regulatory determinant of mitochondrial Complex III assembly in human cells.; Authors - Maxence Le Vasseur
mouse	WB,IF	Environ Pollut; Fluoride induced mitochondrial impairment and PINK1-mediated mitophagy in Leydig cells of mice: In vivo and in vitro studies.; Authors - Chen Liang
human	WB,IF	Viruses; Interaction between PHB2 and Enterovirus A71 VP1 Induces Autophagy and Affects EV-A71 Infection.; Authors - Weitao Su ; KD Validated
human	IHC	Mol Biol Cell; Discovery of bactericides as an acute mitochondrial membrane damage inducer.; Authors - Ryan Houston
human	IF	PLoS One; A549 cells contain enlarged mitochondria with independently functional clustered mtDNA nucleoids.; Authors - Aleksandrs Nasonovs