Anticorps Monoclonal anti-Podoplanin

Podoplanin Monoclonal Antibody for IHC,ELISA

Hôte / Isotype

Mouse / IgG2a

Réactivité testée

Humain

Applications

WB, IHC, ELISA

Conjugaison

Non conjugué

Publications

3

CloneNo.

1D9F3

N° de cat : 67432-1-Ig

Synonymes

AGGRUS, D2 40, D2 40,M2A, D240, Glycoprotein 36, GP36, Gp38, GP40, HT1A 1, M2A, OTS8, PA2.26, PA2.26 antigen, PDPN, podoplanin, PSEC0025, T1 alpha, T1A, T1A 2

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Galerie de données de validation

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  IHC staining of human tonsillitis using 67432-1-Ig

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 67432-1-Ig (Podoplanin antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human tonsillitis using 67432-1-Ig

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 67432-1-Ig (Podoplanin antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon using 67432-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 67432-1-Ig (Podoplanin antibody) at dilution of 1:10000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon using 67432-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 67432-1-Ig (Podoplanin antibody) at dilution of 1:10000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human appendicitis using 67432-1-Ig

  Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 67432-1-Ig (PDPN antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human appendicitis using 67432-1-Ig

  Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 67432-1-Ig (Podoplanin antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human appendicitis using 67432-1-Ig

  Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 67432-1-Ig (Podoplanin antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en IHC: tissu d'amygdalite humain, tissu d'appendicite humain, tissu de côlon humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Immunohistochimie (IHC): IHC : 1:2000-1:8000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 1 publications below
• IHC: See 2 publications below

Informations sur le produit

67432-1-Ig cible Podoplanin dans les applications de WB, IHC, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain
• Hôte / Isotype: Mouse / IgG2a
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: Podoplanin Protéine recombinante Ag17691
• Nom complet: podoplanin
• Masse moléculaire calculée: 238 aa, 25 kDa
• Numéro d’acquisition GenBank: BC022812
• Symbole du gène: PDPN
• Identification du gène (NCBI): 10630
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20 ℃. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Podoplanin was identified as a glycoprotein found in the cell membranes of glomerular epithelial cells (podocyte) (PMID: 9327748). It is a lymphatic marker because the expression of podoplanin has been detected in lymphatic but not blood vascular endothelium, and is useful as the marker of tumor-associated Lymphangiogenesis. Podoplanin has a function in developing testis, most likely at the level of cell-cell interactions among pre-meiotic germ cells and immature Sertoli cells. It may be involved in cell migration and/or actin cytoskeleton organization. When expressed in keratinocytes, PDPN induces changes in cell morphology with transfected cells showing an elongated shape, numerous membrane protrusions, major reorganization of the actin cytoskeleton, increased motility and decreased cell adhesion. It is required for normal lung cell proliferation and alveolus formation at birth. PDPN induces platelet aggregation. It does not have any effect on folic acid or amino acid transport and does not function as a water channel or as a regulator of aquaporin-type water channels. The antibody is specific to Podoplanin.

Publications

Species	Application	Title
human	IHC	Am J Pathol; SET domain-containing 5 (SETD5) facilitates tumor growth and pulmonary metastasis through up-regulation of AKT1 signaling in breast cancer.; Authors - Zhaoting Yang
human	IHC	Cell Death Dis; FASN promotes lymph node metastasis in cervical cancer via cholesterol reprogramming and lymphangiogenesis.; Authors - Qiqiao Du
human	WB	Drug Resist Updat; PDPN positive CAFs contribute to HER2 positive breast cancer resistance to trastuzumab by inhibiting antibody-dependent NK cell-mediated cytotoxicity; Authors - Ruoxin Du