Anticorps Monoclonal anti-Gamma Catenin

Gamma Catenin Monoclonal Antibody for IF, IHC, WB, ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

canin, Humain, porc, rat, souris

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

2

CloneNo.

1A5D10

Avis (1)

N° de cat : 66445-1-Ig

Synonymes

ARVD12, Catenin gamma, CTNNG, Desmoplakin 3, Desmoplakin III, DP3, DPIII, junction plakoglobin, JUP, PDGB, PKGB, γ-Catenin

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Galerie de données de validation

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  WB analysis of human heart using 66445-1-Ig

  human heart tissue were subjected to SDS PAGE followed by western blot with 66445-1-Ig (Plakoglobin/gamma catenin antibody at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat heart using 66445-1-Ig

  rat heart tissue were subjected to SDS PAGE followed by western blot with 66445-1-Ig (Plakoglobin/gamma catenin antibody at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HT-29 using 66445-1-Ig

  HT-29 cells were subjected to SDS PAGE followed by western blot with 66445-1-Ig (gamma catenin antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of T-47D using 66445-1-Ig

  T-47D cells were subjected to SDS PAGE followed by western blot with 66445-1-Ig (gamma catenin antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A431 using 66445-1-Ig

  A431 cells were subjected to SDS PAGE followed by western blot with 66445-1-Ig (gamma catenin antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human heart using 66445-1-Ig

  human heart tissue were subjected to SDS PAGE followed by western blot with 66445-1-Ig (gamma catenin antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat heart using 66445-1-Ig

  rat heart tissue were subjected to SDS PAGE followed by western blot with 66445-1-Ig (gamma catenin antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MDCK using 66445-1-Ig

  MDCK cells were subjected to SDS PAGE followed by western blot with 66445-1-Ig (gamma catenin antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human breast cancer using 66445-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66445-1-Ig (gamma catenin antibody) at dilution of 1:2000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 66445-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66445-1-Ig (gamma catenin antibody) at dilution of 1:2000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat stomach using 66445-1-Ig

  Immunohistochemical analysis of paraffin-embedded rat stomach tissue slide using 66445-1-Ig (Gamma Catenin antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat stomach using 66445-1-Ig

  Immunohistochemical analysis of paraffin-embedded rat stomach tissue slide using 66445-1-Ig (Gamma Catenin antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse stomach using 66445-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse stomach tissue slide using 66445-1-Ig (Gamma Catenin antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse stomach using 66445-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse stomach tissue slide using 66445-1-Ig (Gamma Catenin antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human stomach cancer using 66445-1-Ig

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 66445-1-Ig (Gamma Catenin antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human stomach cancer using 66445-1-Ig

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 66445-1-Ig (Gamma Catenin antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human skin using 66445-1-Ig

  Immunohistochemical analysis of paraffin-embedded human skin tissue slide using 66445-1-Ig (Plakoglobin/gamma catenin antibody at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human skin using 66445-1-Ig

  Immunohistochemical analysis of paraffin-embedded human skin tissue slide using 66445-1-Ig (Plakoglobin/gamma catenin antibody at dilution of 1:200 (under 40x lens).

• 

  IF Staining of A431 using 66445-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using Gamma Catenin antibody (66445-1-Ig, Clone: 1A5D10 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

• 

  IF Staining of MCF-7 using 66445-1-Ig

  Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using Gamma Catenin antibody (66445-1-Ig, Clone: 1A5D10 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Applications testées

• Résultats positifs en WB: tissu cardiaque humain, cellules A431, cellules HT-29, cellules MDCK, cellules T-47D, tissu cardiaque de rat
• Résultats positifs en IHC: tissu de cancer du sein humain, tissu cutané humain, tissu de cancer de l'estomac humain, tissu d'estomac de rat, tissu d'estomac de souris; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules A431, cellules MCF-7

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochimie (IHC): IHC : 1:1000-1:4000
• Immunofluorescence (IF): IF : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 2 publications below

Informations sur le produit

66445-1-Ig cible Gamma Catenin dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons canin, Humain, porc, rat, souris

• Réactivité: canin, Humain, porc, rat, souris
• Réactivité citée: Humain
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: Gamma Catenin Protéine recombinante Ag1627
• Nom complet: junction plakoglobin
• Masse moléculaire calculée: 82 kDa
• Poids moléculaire observé: 82 kDa
• Numéro d’acquisition GenBank: BC011865
• Symbole du gène: JUP
• Identification du gène (NCBI): 3728
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Plakoglobin (γ-catenin), a member of the Armadillo proteins, plays important roles in cell adhesion with α-catenin and β-catenin, is involved in Wnt signaling, and is translocated into the nucleus and binds LEF1. Plakoglobin is a major cytoplasmic component of both desmosomes and adherens junctions, and is the only known constituent common to submembranous plaques in both of these structures, which are located at the intercalated disc (ICD) of cardiomyocytes. Plakoglobin links cadherins to the actin cytoskeleton. Mutations in plakoglobin are associated with arrhythmogenic right ventricular dysplasia and Pemphigus vulgaris.

Publications

Species	Application	Title
human	WB	Aging (Albany NY); DSC2 suppresses the growth of gastric cancer through the inhibition of nuclear translocation of γ-catenin and PTEN/PI3K/AKT signaling pathway; Authors - Chao Sun
human	WB	Oxid Med Cell Longev; DSC2 Suppresses the Metastasis of Gastric Cancer through Inhibiting the BRD4/Snail Signaling Pathway and the Transcriptional Activity of β-Catenin; Authors - Chao Sun

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Tom (Verified Customer) (11-02-2023)

Multiple bands and high background in western blot (1:2000, overnight incubation at 4C in 3% BSA PBS 0.1% Tween on PVDF membrane). Non-specific (and non-junctional) signalling in immunoflouresence (1:200, 4% PFA fixed cells).

• Applications: Western Blot, Immunofluorescence
• Primary Antibody Dilution: 1:2000 (WB) , 1:200 (IF)
• Cell Tissue Type: HUVEC

Gamma Catenin Antibody Western Blot,Immunofluorescence validation (1:2000 (WB) , 1:200 (IF) dilution) in HUVEC (Cat no:66445-1-Ig)