Anticorps Polyclonal de lapin anti-Phospholipase C epsilon 1

Phospholipase C epsilon 1 Polyclonal Antibody for IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

IHC, ELISA

Conjugaison

Non conjugué

N° de cat : 55463-1-AP

Synonymes

KIAA1516, Phospholipase C epsilon 1, phospholipase C, epsilon 1, PLC epsilon 1, PLCE, PLCE1, PPLC

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Galerie de données de validation

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  IHC staining of human kidney using 55463-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 55463-1-AP (Phospholipase C epsilon 1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human kidney using 55463-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 55463-1-AP (Phospholipase C epsilon 1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung cancer using 55463-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 55463-1-AP (Phospholipase C epsilon 1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 55463-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 55463-1-AP (Phospholipase C epsilon 1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon using 55463-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 55463-1-AP (Phospholipase C epsilon 1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human oesophagus cancer using 55463-1-AP

  Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using 55463-1-AP (Phospholipase C epsilon 1 antibody at dilution of 1:100 (under 10x lens).

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  IHC staining of human oesophagus cancer using 55463-1-AP

  Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using 55463-1-AP (Phospholipase C epsilon 1 antibody at dilution of 1:100 (under 40x lens).

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  IHC staining of human stomach cancer using 55463-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 55463-1-AP (Phospholipase C epsilon 1 antibody at dilution of 1:100 (under 10x lens).

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  IHC staining of human stomach cancer using 55463-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 55463-1-AP (Phospholipase C epsilon 1 antibody at dilution of 1:100 (under 40x lens).

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Applications testées

• Résultats positifs en IHC: tissu rénal humain, tissu de cancer de l'estomac humain, tissu de cancer de l'œsophage humain, tissu de cancer du côlon humain, tissu de cancer du poumon humain, tissu de côlon humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Immunohistochimie (IHC): IHC : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

55463-1-AP cible Phospholipase C epsilon 1 dans les applications de IHC, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: phospholipase C, epsilon 1
• Masse moléculaire calculée: 257 kDa
• Numéro d’acquisition GenBank: NM_016341
• Symbole du gène: PLCE1
• Identification du gène (NCBI): 51196
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Phospholipase C epsilon 1 (PLCE1) is also named as KIAA1516, PLCE and PPLC. TRAPPC10 (Cyclin-J) Belongs to the Cyclin family. PLCE1, which mediates small GTPases of the Ras superfamily through the activity of its Ras guanine exchange factor, is a bifunctional enzyme (PMID: 31867717). As the effector of heterotrimer and small G protein, it is involved in regulating cell growth, cell survival, T-cell activation, and actin organization (PMID: 32238860). PLCE1 is identified as a member of phospholipase family, and PLCE1 was upregulated in LPS-induced pneumonia cells (PMID: 35527658). PLCE1 is involved in the regulation of Rho GTPases and MAPKs in podocytes, and PLCE1 interacts with NCK2 but not with NCK1 (PMID: 32238860). PLCE1 is a multifunctional enzyme exhibiting phospholipase as well as RasGEF activities (PMID: 11022047).