Anticorps Polyclonal de lapin anti-Phospho-p70(S6K) (Thr389)
Phospho-p70(S6K) (Thr389) Polyclonal Antibody for WB, ELISA
Hôte / Isotype
Lapin / IgG
Réactivité testée
Humain et plus (1)
Applications
WB, ELISA
Conjugaison
Non conjugué
N° de cat : 28735-1-AP
Synonymes
Galerie de données de validation
Applications testées
Résultats positifs en WB | cellules MCF-7 traitées à l'IGF-1 |
Dilution recommandée
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:1000-1:8000 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, check data in validation data gallery |
Applications publiées
WB | See 16 publications below |
Informations sur le produit
28735-1-AP cible Phospho-p70(S6K) (Thr389) dans les applications de WB, ELISA et montre une réactivité avec des échantillons Humain
Réactivité | Humain |
Réactivité citée | rat, Humain |
Hôte / Isotype | Lapin / IgG |
Clonalité | Polyclonal |
Type | Anticorps |
Immunogène | Peptide |
Nom complet | ribosomal protein S6 kinase, 70kDa, polypeptide 1 |
Poids moléculaire observé | 65-85 kDa |
Numéro d’acquisition GenBank | NM_003161 |
Symbole du gène | RPS6KB1 |
Identification du gène (NCBI) | 6198 |
Conjugaison | Non conjugué |
Forme | Liquide |
Méthode de purification | Purification par affinité contre l'antigène |
Tampon de stockage | PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3 |
Conditions de stockage | Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20oC Les 20ul contiennent 0,1% de BSA. |
Informations générales
The Rps6kb1 gene encodes the 70 kDa ribosomal protein S6 kinase (p70S6K), which is a serine/threonine kinase regulated by phosphoinositide 3-kinase (PI3K)/mammalian target of rapamycin (mTOR) pathway. P70S6K plays a crucial role in controlling cell cycle, growth and survival. The PI3K/mTOR signalling pathway is one of the major mechanisms for controlling cell survival, proliferation and metabolism and is the central regulator of translation of some components of protein synthesis system. Due to alternative translation two isoform S6K1 proteins are known to exist in mammalian cells: p85 S6K1 and p70 S6K1, which is identical to p85 S6K but lacks its first 23 amino acids. In addition, mammalian cells express a second S6K1 isoform spanning 316 amino acids (p31 S6K1). mTOR is know to phosphorylate and thereby activate p70 S6K1 at T389 and p85 S6K1 at T412. (PMID: 25100792, PMID: 24970012, PMID: 21602892)
Protocole
Product Specific Protocols | |
---|---|
WB protocol for Phospho-p70(S6K) (Thr389) antibody 28735-1-AP | Download protocol |
Standard Protocols | |
---|---|
Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Cell Death Differ Lysine methylation of PPP1CA by the methyltransferase SUV39H2 disrupts TFEB-dependent autophagy and promotes intervertebral disc degeneration | ||
Oxid Med Cell Longev Alpha-Ketoglutarate Alleviates Neuronal Apoptosis Induced by Central Insulin Resistance through Inhibiting S6K1 Phosphorylation after Subarachnoid Hemorrhage | ||
Aging (Albany NY) UPF1 impacts on mTOR signaling pathway and autophagy in endometrioid endometrial carcinoma. | ||
Int J Mol Sci Effects of Cynaroside on Cell Proliferation, Apoptosis, Migration and Invasion though the MET/AKT/mTOR Axis in Gastric Cancer. | ||
Cell Signal Autophagy activation attenuates the circadian clock oscillators in U2OS cells via the ATG5 pathway |