Anticorps Recombinant de lapin anti-Phospho-TBK1 (Ser172)

Phospho-TBK1 (Ser172) Recombinant Antibody for WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain et plus (1)

Applications

WB, ELISA

Conjugaison

Non conjugué

Publications

2

CloneNo.

2K2

Avis (1)

N° de cat : 82383-1-RR

Synonymes

FLJ11330, NAK, NF kappa B activating kinase, Phospho-TBK1 (Ser172), T2K, TANK binding kinase 1, TBK1

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Galerie de données de validation

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  WB analysis of HeLa using 82383-1-RR

  Non-treated and Calyculin A treated HeLa cells were subjected to SDS PAGE followed by western blot with 82383-1-RR (Phospho-TBK1 (Ser172) antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Alpha Tubulin antibody as loading control.

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Applications testées

• Résultats positifs en WB: cellules HeLa traitées à la calyculine A,

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 2 publications below

Informations sur le produit

82383-1-RR cible Phospho-TBK1 (Ser172) dans les applications de WB, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: TANK-binding kinase 1
• Poids moléculaire observé: 84 kDa
• Numéro d’acquisition GenBank: BC034950
• Symbole du gène: TBK1
• Identification du gène (NCBI): 29110
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

TBK1, also named as tumor necrosis factor (TNF) receptor-associated factor NF-kB activator (TANK)-binding kinase 1 (TBK1), NF-kB-activating kinase (NAK), T2K, is a multimeric kinase that modulates inflammation and autophagy. It is a ubiquitously expressed serine-threonine kinase belonging to the 'noncanonical IkB kinases' (IKKs) recognized for its critical role in regulating type I IFN production (PMID: 27211305). And TBK1 is an important player in yet another critical cellular function, autophagy.

Publications

Species	Application	Title
human,mouse	WB	Signal Transduct Target Ther; The cGAS-STING pathway-dependent sensing of mitochondrial DNA mediates ocular surface inflammation; Authors - Weijie Ouyang
mouse	WB	Eur J Pharmacol; Nitisinone attenuates cartilage degeneration and subchondral osteoclastogenesis in osteoarthritis and concomitantly inhibits the cGAS/STING/NF-κB pathway; Authors - Tao Yang

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Marina (Verified Customer) (09-27-2023)

Western blot using p-TBK1 antibody at 1:5000 dilution overnight at 4ºC. Samples are two breast cancer (BC) cell lines, ran in duplicate. Ponceau staining is shown as total protein loading control. A more prominent band can be seen below 100 kDa, and after more exposure, another band at around 80 kDa can be observed.

• Applications: Western Blot
• Primary Antibody Dilution: 1:5000
• Cell Tissue Type: Triple negative breast cancer cell lines

Phospho-TBK1 (Ser172) Antibody Western Blot validation (1:5000 dilution) in Triple negative breast cancer cell lines (Cat no:82383-1-RR)