Anticorps Polyclonal de lapin anti-Phospho-STK11/LKB1 (Thr189)

Phospho-STK11/LKB1 (Thr189) Polyclonal Antibody for WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, ELISA

Conjugaison

Non conjugué

Publications

1

N° de cat : 28965-1-AP

Synonymes

LKB1, Phospho-STK11 (Thr189), PJS, serine/threonine kinase 11, STK11

---

Galerie de données de validation

• 

  WB analysis using 28965-1-AP

  Non-treated PC-3 and Calyculin A treated PC-3 cells were subjected to SDS PAGE followed by western blot with 28965-1-AP (Phospho-STK11/LKB1 (Thr189) antibody) at dilution of 1:10000 incubated at room temperature for 1 hours.

• 

  WB analysis using 28965-1-AP

  Non-treated and UV treated NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 28965-1-AP (Phospho-STK11/LKB1 (Thr189) antibody) at dilution of 1:1000 incubated at 4℃ overnight.

---

Applications testées

• Résultats positifs en WB: cellules PC-3 traitées à la calyculine A, cellules NIH/3T3 traitées au UV

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 1 publications below

Informations sur le produit

28965-1-AP cible Phospho-STK11/LKB1 (Thr189) dans les applications de WB, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: serine/threonine kinase 11
• Masse moléculaire calculée: 49 kDa
• Poids moléculaire observé: 50-55 kDa
• Numéro d’acquisition GenBank: BC007981
• Symbole du gène: STK11
• Identification du gène (NCBI): 6794
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20 ℃. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Liver kinase 1 (LKB1 also known as STK11) is a tumor suppressor gene that is inactivated by bi-allelic mutation in non-small cell lung cancer (NSCLC), malignant melanoma and cervical cancer. LKB1 protein has a central kinase domain, two N-terminal nuclear leading sequences, and a C-terminal regulatory motif. The protein also contains a CAAX motif at C-terminal end and can be farnesylated. The formation of an LKB1/Strad/MO25 complex is essential for LKB1's kinase activity, and there are at least 13 known substrates of LKB1, which includes AMPKs, BRSKs, and MARKs. LKB1 is the major kinase that phosphorylates the AMPK activation loop in conditions of energy stress. (PMID: 25679014, PMID: 19629071)

Publications

Species	Application	Title
human	WB	Cells; Psoralen Suppresses Lipid Deposition by Alleviating Insulin Resistance and Promoting Autophagy in Oleate-Induced L02 Cells.; Authors - Yuhao Wang